Golgi Distribution of Lyn to Caveolin- and Giantin-Positive cis-Golgi Membranes and the Caveolin-Negative, TGN46-Positive trans-Golgi Network

• Okamoto Aya

  Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University

• Morinaga Takao

  Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University Division of Pathology and Cell Therapy, Chiba Cancer Center Research Institute

• Yamaguchi Noritaka

  Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University

• Yamaguchi Naoto

  Laboratory of Molecular Cell Biology, Graduate School of Pharmaceutical Sciences, Chiba University

書誌事項

タイトル別名

• Golgi Distribution of Lyn to Caveolin- and Giantin-Positive <i>cis</i>-Golgi Membranes and the Caveolin-Negative, TGN46-Positive <i>trans</i>-Golgi Network

抄録

Src-family tyrosine kinases, classified as cytosolic enzymes, have crucial roles in regulating cell proliferation, differentiation, migration and cell-shape changes. Newly synthesized Lyn, a member of Src-family kinases, is biosynthetically accumulated at the cytoplasmic face of caveolin-containing Golgi membranes via posttranslational lipid modifications and then transported to the plasma membrane. However, the precise intra-Golgi localization of Lyn remains elusive. By means of a 19°C block-release technique and short-term brefeldin A treatment, we show here that the distribution of Lyn is not monotonously spread within the Golgi but selectively intensified in two distinct membrane compartments: giantin- and caveolin-positive membranes and trans-Golgi network protein (TGN)46-positive but caveolin-negative membranes. Furthermore, Lyn exits the Golgi from the caveolin-positive cis-Golgi cisternae or the caveolin-negative trans-Golgi network. These results suggest that Lyn moves apart from caveolin, a secretory protein, within the Golgi during Lyn’s trafficking to the plasma membrane.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 41 (1), 142-146, 2018

  公益社団法人 日本薬学会

キーワード

• Lyn
• Golgi
• trafficking
• caveolin
• giantin
• <i>trans</i>-Golgi network protein (TGN)46

詳細情報

• CRID: 1390001204634422144
• NII論文ID: 130006301302
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.b17-00681
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 028739501
• PubMed: 29311477
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028739501
• Web Site: https://www.jstage.jst.go.jp/article/bpb/41/1/41_b17-00681/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018321723
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可