Protective effects of <i>Phaseolus vulgaris</i> lectin against viral infection in <i>Drosophila </i>

• Ekowati Heny

  Graduate School of Medical Sciences, Kanazawa University Faculty of Health Science, Jenderal Soedirman University

• Arai Junko

  Product Development Laboratory, J-Oil Mills, Inc.

• Damana Putri Ayu Sekarani

  Graduate School of Medical Sciences, Kanazawa University Faculty of Medicine, Brawijaya University

• Nainu Firzan

  Faculty of Pharmacy, Hasanuddin University

• Shiratsuchi Akiko

  Graduate School of Medical Sciences, Kanazawa University

• Nakanishi Yoshinobu

  Graduate School of Medical Sciences, Kanazawa University

Bibliographic Information

Other Title

• Protective effects of Phaseolus vulgaris lectin against viral infection in Drosophila

Abstract

Phytohemagglutinin (PHA) isolated from the family of Phaseolus vulgaris beans is a promising agent against viral infection; however, it has not yet been demonstrated in vivo. We herein investigated this issue using Drosophila as a host. Adult flies were fed lectin approximately 12 h before they were subjected to a systemic viral infection. After a fatal infection with Drosophila C virus, death was delayed and survival was longer in flies fed PHA-P, a mixture of L4, L3E1, and L2E2, than in control unfed flies. We then examined PHA-L4, anticipating subunit L as the active form, and confirmed the protective effects of this lectin at markedly lower concentrations than PHA-P. In both experiments, lectin feeding reduced the viral load prior to the onset of fly death. Furthermore, we found a dramatic increase in the levels of the mRNAs of phagocytosis receptors in flies after feeding with PHA-L4 while a change in the levels of the mRNAs of antimicrobial peptides was marginal. We concluded that P. vulgaris PHA protects Drosophila against viral infection by augmenting the level of host immunity.

Journal

• Drug Discoveries & Therapeutics

  Drug Discoveries & Therapeutics 11 (6), 329-335, 2017

  International Research and Cooperation Association for Bio & Socio-Sciences Advancement

Keywords

• Antiviral effect
• innate immunity
• plant lectin

Details

• CRID: 1390282680714664064
• NII Article ID: 130006308080
• DOI: 10.5582/ddt.2017.01071
• ISSN: 1881784X
• ISSN: 18817831
• PubMed: 29332891
• Web Site: https://www.jstage.jst.go.jp/article/ddt/11/6/11_2017.01071/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018321763
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed