Rapid and Accurate Diagnosis Based on Real-Time PCR Cycle Threshold Value for the Identification of <i>Campylobacter jejuni</i>, <i>astA</i> Gene-Positive <i>Escherichia coli</i>, and <i>eae</i> Gene-Positive <i>E. coli</i>
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- Kawase Jun
- Shimane Prefectural Institute of Public Health and Environmental Science
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- Asakura Hiroshi
- Division of Biomedical Food Research, National Institute of Health Sciences
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- Kurosaki Morito
- Shimane Prefectural Institute of Public Health and Environmental Science
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- Oshiro Hitoshi
- Shimane Prefectural Institute of Public Health and Environmental Science
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- Etoh Yoshiki
- Fukuoka Institute of Health and Environmental Sciences
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- Ikeda Tetsuya
- Hokkaido Institute of Public Health
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- Watahiki Masanori
- Toyama Institute of Health
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- Kameyama Mitsuhiro
- Yamaguchi Prefectural Institute of Public Health and Environment
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- Hayashi Fumi
- Shimane Prefectural Institute of Public Health and Environmental Science
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- Kawakami Yuta
- Shimane Prefectural Institute of Public Health and Environmental Science
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- Murakami Yoshiko
- Shimane Prefectural Institute of Public Health and Environmental Science
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- Tsunomori Yoshie
- Shimane Prefectural Institute of Public Health and Environmental Science
Bibliographic Information
- Other Title
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- Rapid and Accurate Diagnosis Based on Real-Time PCR Cycle Threshold Value for the Identification of Campylobacter jejuni, astA Gene-Positive Escherichia coli, and eae Gene-Positive E. coli
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Abstract
<p>We previously developed a multiplex real-time PCR assay (Rapid Foodborne Bacterial Screening 24 ver.5, [RFBS24 ver.5]) for simultaneous detection of 24 foodborne bacterial targets. Here, to overcome the discrepancy of the results from RFBS24 ver.5 and bacterial culture methods (BC), we analyzed 246 human clinical samples from 49 gastroenteritis outbreaks using RFBS24 ver.5 and evaluated the correlation between the cycle threshold (CT) value of RFBS24 ver.5 and the BC results. The results showed that the RFBS24 ver.5 was more sensitive than BC for Campylobacter jejuni and Escherichia coli harboring astA or eae, with positive predictive values (PPV) of 45.5–87.0% and a kappa coefficient (KC) of 0.60–0.92, respectively. The CTs were significantly different between BC-positive and -negative samples (p < 0.01). All RFBS24 ver.5-positive samples were BC-positive under the lower confidence interval (CI) limit of 95% or 99% for the CT of the BC-negative samples. We set the 95% or 99% CI lower limit to the determination CT (d-CT) to discriminate for assured BC-positive results (d-CTs: 27.42–30.86), and subsequently the PPVs (94.7%–100.0%) and KCs (0.89–0.95) of the 3 targets were increased. Together, we concluded that the implication of a d-CT-based approach would be a valuable tool for rapid and accurate diagnoses using the RFBS24 ver.5 system.</p>
Journal
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 71 (1), 79-84, 2018
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
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Details 詳細情報について
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- CRID
- 1390282681217312896
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- NII Article ID
- 130006317361
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- NII Book ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL BIB ID
- 028792673
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- PubMed
- 29093317
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed