A novel compensating wheat–<i>Thinopyrum elongatum</i> Robertsonian translocation line with a positive effect on flour quality

• Tanaka Hiroyuki

  Laboratory of Plant Genetics, Faculty of Agriculture, Tottori University

• Nabeuchi Chisato

  Laboratory of Plant Genetics, Faculty of Agriculture, Tottori University

• Kurogaki Misaki

  Laboratory of Plant Genetics, Faculty of Agriculture, Tottori University

• Garg Monika

  National Agri-food Biotechnology Institute

• Saito Mika

  NARO Tohoku National Agriculture Research Center

• Ishikawa Goro

  NARO Tohoku National Agriculture Research Center NARO Institute of Crop Science

• Nakamura Toshiki

  NARO Tohoku National Agriculture Research Center

• Tsujimoto Hisashi

  Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University

Bibliographic Information

Other Title

• A novel compensating wheat-Thinopyrum elongatum Robertsonian translocation line with a positive effect on flour quality

Abstract

Wheat flours are used to produce bread, pasta, breakfast cereals, and biscuits; the various properties of these end-products are attributed to the gluten content, produced as seed storage proteins in the wheat endosperm. Thus, genes encoding gluten protein are major targets of wheat breeders aiming to improve the various properties of wheat flour. Here, we describe a novel compensating wheat–Thinopyrum elongatum Robertsonian translocation (T1AS.1EL) line involving the short arm of wheat chromosome 1A (1AS) and the long arm of Th. elongatum chromosome 1E (1EL); we developed this line through centric breakage-fusion. Compared to the common wheat cultivars Chinese Spring and Norin 61, we detected two additional 1EL-derived high-molecular-weight glutenin subunits (HMW-GSs) in the T1AS.1EL plants. Based on the results of an SDS-sedimentation volume to estimate the gluten strength of T1AS.1EL-derived flour, we predict that T1AS.1EL-derived flour is better suited to bread-making than Chinese Spring- and Norin 61-derived flour and that this is because of its greater gluten diversity. Also, we were able to assign 33 of 121 wheat PCR-based Landmark Unique Gene markers to chromosome 1E of Th. elongatum. These markers can now be used for further chromosome engineering of the Th. elongatum segment of T1AS.1EL.

Journal

• Breeding Science

  Breeding Science 67 (5), 509-517, 2017

  Japanese Society of Breeding

Keywords

• <i>Triticum aestivum</i>
• <i>Thinopyrum elongatum</i>
• Robertsonian translocation
• bread-making quality
• wheat PLUG markers

Details

• CRID: 1390282679698724992
• NII Article ID: 130006318320
• NII Book ID: AA11317194
• DOI: 10.1270/jsbbs.17058
• ISSN: 13473735
• ISSN: 13447610
• NDL BIB ID: 028736943
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028736943
• Web Site: https://www.jstage.jst.go.jp/article/jsbbs/67/5/67_17058/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed