Hybrid origin of Asian aspermic <i>Fasciola</i> flukes is confirmed by analyzing two single-copy genes, <i>pepck</i> and <i>pold</i>
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- HAYASHI Kei
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan
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- ICHIKAWA-SEKI Madoka
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan
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- MOHANTA Uday Kumar
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan Department of Microbiology and Parasitology, Faculty of Animal Science and Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh
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- SHORIKI Takuya
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan
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- CHAICHANASAK Pannigan
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan Faculty of Veterinary Medicine, Mahanakorn University of Technology, 140 Cheum-Sampan Road, Nong Chok, Bangkok 10530, Thailand
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- ITAGAKI Tadashi
- Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan
Bibliographic Information
- Other Title
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- Hybrid origin of Asian aspermic Fasciola flukes is confirmed by analyzing two single-copy genes, pepck and pold
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Abstract
<p>Nuclear gene markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), have been developed for precise discrimination of Fasciola flukes instead of internal transcribed spacer 1. In this study, these two genes of 730 Fasciola flukes from eight Asian countries were analyzed. The results were compared with their mitochondrial NADH dehydrogenase subunit 1 (nad1) lineages for obtaining a definitive evidence of the hybrid origin of aspermic Fasciola flukes. All the flukes categorized into the aspermic nad1 lineages possessed both the fragment patterns of F. hepatica and F. gigantica (mixed types) in pepck and/or pold. These findings provide clear evidence for the hybrid origin of aspermic Fasciola lineages and suggest that “aspermic Fasciola flukes” should hereafter be called “hybrid Fasciola flukes”.</p>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 80 (1), 98-102, 2018
JAPANESE SOCIETY OF VETERINARY SCIENCE