Recovery of <i>Bordetella bronchiseptica</i> sequence type 82 and <i>B. pseudohinzii</i> from urban rats in Terengganu, Malaysia
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- LOONG Shih Keng
- Tropical Infectious Diseases Research & Education Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
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- CHE-MAT-SERI Nurul-Asma-Anati
- Tropical Infectious Diseases Research & Education Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
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- ABDULRAZAK Osama
- Microbial Evolutionary Dynamics Research Group, Department of Evolutionary Theory, Max Planck Institute for Evolutionary Biology, 24306 Plön, Germany
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- DOUADI Benacer
- Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
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- AHMAD-NASRAH Siti-Noraisah
- Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
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- JOHARI Jefree
- Tropical Infectious Diseases Research & Education Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
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- MOHD-ZAIN Siti-Nursheena
- Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
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- ABUBAKAR Sazaly
- Tropical Infectious Diseases Research & Education Centre, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
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Abstract
<p>Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mild to severe respiratory disease in mammals and, some have been described to infect, colonize and spread in rodents. There is a lack of information on the population diversity of bordetellae among Malaysian wild rodents. Here, bordetellae recovered from lung tissues of wild rats were genotypically characterized using 16S rDNA sequencing, MLST and nrdA typing. A novel B. bronchiseptica ST82, closely related to other human-derived isolates, was discovered in three wild rats (n=3) from Terengganu (5.3333° N, 103.1500° E). B. pseudohinzii, a recently identified laboratory mice inhabitant, was also recovered from one rat (n=1). Both bordetellae displayed identical antimicrobial resistance profiles, indicating the close phylogenetic association between them. Genotyping using the 765-bp nrdA locus was shown to be compatible with the MLST-based phylogeny, with the added advantage of being able to genotype non-classical bordetellae. The recovery of B. pseudohinzii from wild rat implied that this bordetellae has a wider host range than previously thought. The findings from this study suggest that bordetellae surveillance among wild rats in Malaysia has to be continued and expanded to other states to ensure early identification of species capable of causing public health disorder.</p>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 80 (1), 77-84, 2018
JAPANESE SOCIETY OF VETERINARY SCIENCE
