Magnetic structure of manganese cluster in photosystem II investigated by electron paramagnetic resonance

• Nagashima Hiroki

  Division of Material Science (Physics), Graduate School of Science, Nagoya University Present address: Laser Molecular Photoscience Laboratory, Molecular Photoscience Research Center, Kobe University

• Asada Mizue

  Division of Material Science (Physics), Graduate School of Science, Nagoya University Present address: Department of Materials Molecular Science, Institute for Molecular Science

• Mino Hiroyuki

  Division of Material Science (Physics), Graduate School of Science, Nagoya University

Abstract

The electronic structure of manganese (Mn) cluster in photosystem II was investigated by electron paramagnetic resonance (EPR) spectroscopy. In order to determine the spin density distribution in magnetically coupled Mn in the S₂ state Mn cluster, pulsed electron–electron double resonance (PELDOR) measurement was performed. The local environment of the Mn cluster was investigated by electron-nuclear double resonance (ENDOR). Using spin projections determined by PELDOR, ENDOR signals were assigned to the water molecules ligated to the Mn cluster. The location of a high-affinity Mn²⁺ site in apo-photosystem II, which is the initial site of photoactivation of the Mn cluster, was determined by PELDOR.

Journal

• Biophysics and Physicobiology

  Biophysics and Physicobiology 15 (0), 45-50, 2018

  The Biophysical Society of Japan

Keywords

• photosystem II
• manganese cluster
• EPR
• PELDOR
• ENDOR

Details

• CRID: 1390001205764878336
• NII Article ID: 130006337977
• DOI: 10.2142/biophysico.15.0_45
• ISSN: 21894779
• Web Site: https://www.jstage.jst.go.jp/article/biophysico/15/0/15_45/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed