Determination of chemical-specific IgGs in serum by an enzyme-linked immunosorbent assay with partial peptides of human serum albumin

• Ishihara Yasuhiro

  Laboratory of Molecular Brain Science, Graduate School of Integrated Arts and Sciences, Hiroshima University Center for Health and the Environment, University of California, USA

• Ikeda-Ishihara Nami

  Division of Gene Research, Natural Science Center for Basic Research and Development, Hiroshima University

• Koriyama Chihaya

  Department of Epidemiology and Preventive Medicine, Kagoshima University Graduate School of Medical and Dental Sciences

• Kakiuchi Noriaki

  Department of Work Systems and Health, Institute of Industrial Ecological Science, University of Occupational and Environmental Health

• Tanaka Masayuki

  Fukuoka Institute of Occupational Health

• Vogel Christoph F.A.

  Center for Health and the Environment, University of California, USA Department of Environmental Toxicology, University of California, USA

• Kawamoto Toshihiro

  Department of Environmental Health, University of Occupational and Environmental Health

• Tsuji Mayumi

  Department of Environmental Health, University of Occupational and Environmental Health

抄録

Many different types of chemicals are used in industry, and occupational allergies are becoming a serious problem in the field of industrial hygiene. In this study, we employed a novel enzyme-linked immunosorbent assay (ELISA) with partial peptides of human serum albumin (HSA) to quantify chemical-specific immunoglobulin G (IgG) in serum for evaluating exposure to chemicals. When HSA partial peptides containing lysine residues were mixed with formaldehyde (FA) or phthalic anhydride (PA), almost all lysine residues were lost. Mass spectrometry revealed that PA and FA formed imine and tertiary amine, respectively, with lysine residues in the peptides. Thus, we used FA- or PA-peptide adducts as an artificial antigen to detect FA- and PA-specific IgGs in serum. The concentrations of FA- and PA-specific IgGs in workers at plants utilizing plastic resins were significantly higher than those in general subjects. This method can estimate exposure levels to chemicals and thus be expected to contribute to the diagnosis of allergies in workers and to the prevention of health hazards due to harmful chemicals.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｔｏｘｉｃｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ 43 (1), 25-31, 2018

  一般社団法人　日本毒性学会

キーワード

• Chemical-specific IgG
• ELISA
• Carbonyl compounds

詳細情報

• CRID: 1390282679881458560
• NII論文ID: 130006338344
• NII書誌ID: AN00002808
• DOI: 10.2131/jts.43.25
• ISSN: 18803989
• ISSN: 03881350
• NDL書誌ID: 029026472
• PubMed: 29415949
• Web Site: http://ir.lib.hiroshima-u.ac.jp/00048688
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029026472
• Web Site: https://www.jstage.jst.go.jp/article/jts/43/1/43_25/_pdf
• 本文言語コード: en
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