Promotion Effect of Streptothricin on a Glucose Oxidase Enzymatic Reaction and Its Application to a Colorimetric Assay

• UEMATSU Kohei

  Department of Bioscience, Fukui Prefectural University

• UENO Takaaki

  Department of Bioscience, Fukui Prefectural University

• KAWASAKI Haruka

  Department of Bioscience, Fukui Prefectural University

• MARUYAMA Chitose

  Department of Bioscience, Fukui Prefectural University

• HAMANO Yoshimitsu

  Department of Bioscience, Fukui Prefectural University

• KATANO Hajime

  Department of Bioscience, Fukui Prefectural University

抄録

Previously, we reported that ε-poly-L-lysine (25 – 35 residues) significantly promoted a glucose oxidase enzymatic (GOx) reaction using ferricyanide ion as the oxidant, and that the effect was due to the formation of a polyion complex between anionic GOx and protonated (polycationic) ε-poly-L-lysine. Here, we show that streptothricins (STs), which have an L-β-lysine oligomer (1 – 7 residues) and possess only several positive charges at most, also effectively promote the GOx enzymatic reaction. Interestingly, the promotion effect increased with the size of the lysine oligomer of STs, suggesting that the ionic valence is a key factor determining the degree of the promotion effect. The GOx enzymatic reaction is accompanied by a color change due to the reduction of yellow ferricyanide ion to a colorless reductant. A more distinctive color change can be achieved by the addition of Fe(III) ions due to the formation of Prussian blue. Thus, the promotion effect allowed for colorimetric detection of STs at the 1 mg/L level. The detection method was simple and easy to carry out, and would become a helpful tool for the detection of STs.

収録刊行物

• Analytical Sciences

  Analytical Sciences 34 (2), 143-148, 2018

  社団法人 日本分析化学会

キーワード

• Multivalent cation
• streptothricin
• promotion effect
• glucose oxidase
• colorimetry

詳細情報

• CRID: 1390282679238007168
• NII論文ID: 130006341964
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.34.143
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 028817982
• PubMed: 29434098
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028817982
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.34.143.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.34.143/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/34/2/34_143/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可