Implementation of Tube Radial Distribution Chromatography by Using a Commercially Available HPLC System

• KAN Hyo

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• YAMADA Kento

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• SANADA Nobuyuki

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• NAKATA Koyo

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• TSUKAGOSHI Kazuhiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University Bio-Microfluidic Science Research Center

Abstract

Tube radial distribution chromatography based on tube radial distribution flow, or annular flow, in an open-tubular capillary has been reported. The chromatographic system requires specific instruments and treatments for microfluidic flow in the capillary tube. In this study, we have developed a new model of tube radial distribution chromatography, which is comprised of a commercially available HPLC system without any packed separation columns. Separation is performed in an open-tubular pipe (100-μm inner diameter and 350-cm length; temperature, 5°C) connected between the pump and the detector in the HPLC system. An analyte solution is introduced with a sample injector (2-μL volume) and a ternary water/acetonitrile/ethyl acetate mixed solution (volume ratio of 3:8:2) is delivered as an eluent solution into the pipe at a flow rate of 10-μL min⁻¹. Fused silica and stainless pipes can separate 1-naphthol and 2,6-naphthalenedisulfonic acid, but a polyetheretherketone pipe cannot. The obtained data provides an important clue to practical developments in separation science.

Journal

• Analytical Sciences

  Analytical Sciences 34 (2), 239-241, 2018

  The Japan Society for Analytical Chemistry

Keywords

• Phase-separation multiphase flow
• tube radial distribution chromatography
• open-tubular pipe
• fused-silica
• stainless

Details

• CRID: 1390282679237972224
• NII Article ID: 130006342046
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.34.239
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 028818226
• PubMed: 29434113
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028818226
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.34.239.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.34.239/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/34/2/34_239/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed