血管中膜におけるコラーゲン/エラスチン線維配向性解明のための定量的解析手法の確立  [in Japanese] Quantitative Analysis of the Preferential Orientation of Collagen and Elastin Fibers in Blood Vessel with an Imaging Methodology Combined with Birefringence Measurement  [in Japanese]

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Author(s)

    • 小笹 良輔 Ozasa Ryosuke
    • 大阪大学大学院工学研究科マテリアル生産科学専攻 Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
    • 永石 武流 Nagaishi Takeru
    • 大阪大学大学院工学研究科マテリアル生産科学専攻 Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
    • 山崎 大介 Yamazaki Daisuke
    • 大阪大学大学院工学研究科マテリアル生産科学専攻 Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
    • 松垣 あいら Matsugaki Aira
    • 大阪大学大学院工学研究科マテリアル生産科学専攻 Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
    • 神崎 万智子 Kanzaki Machiko
    • 大阪大学大学院医学系研究科循環器内科学 Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • 倉谷 徹 Kuratani Toru
    • 大阪大学大学院医学系研究科低侵襲循環器医療学 Department of Minimally Invasive Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • 森井 英一 Morii Eiichi
    • 大阪大学大学院医学系研究科病態病理学 Department of Pathology, Osaka University Graduate School of Medicine
    • 坂田 泰史 Sakata Yasushi
    • 大阪大学大学院医学系研究科循環器内科学 Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • 中野 貴由 Nakano Takayoshi
    • 大阪大学大学院工学研究科マテリアル生産科学専攻 Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

Abstract

<p>Blood vessel exhibits a complicated microstructure depending on the anatomical portion, which enables the realization of its proper mechanical function. In particular, tunica media plays crucial roles in mediating the structural and functional properties of blood vessels. However, the microstructural analysis with quantitative methodology has not been established. In this study, the quantitative analysis of collagen fibers and elastin fibers in tunica media was achieved by combination of a selective staining technique using fluorescent dyes and birefringence measurement with polarized microscopy. The present method quantitatively unveiled the microstructural difference between thoracic aorta and caudal artery. Both types of vessels were comprised of collagen fibers and elastin fibers running along the circumferential direction of tunica media. Interestingly, the degree of preferential orientation of these fibers was markedly increased in thoracic aorta, suggesting that the anisotropic microstructure of vessel varies depending on the anatomical site.</p>

<p>Blood vessel exhibits a complicated microstructure depending on the anatomical portion, which enables the realization of its proper mechanical function. In particular, tunica media plays crucial roles in mediating the structural and functional properties of blood vessels. However, the microstructural analysis with quantitative methodology has not been established. In this study, the quantitative analysis of collagen fibers and elastin fibers in tunica media was achieved by combination of a selective staining technique using fluorescent dyes and birefringence measurement with polarized microscopy. The present method quantitatively unveiled the microstructural difference between thoracic aorta and caudal artery. Both types of vessels were comprised of collagen fibers and elastin fibers running along the circumferential direction of tunica media. Interestingly, the degree of preferential orientation of these fibers was markedly increased in thoracic aorta, suggesting that the anisotropic microstructure of vessel varies depending on the anatomical site.</p>

Journal

  • Journal of the Japan Institute of Metals and Materials

    Journal of the Japan Institute of Metals and Materials 82(3), 64-69, 2018

    The Japan Institute of Metals and Materials

Codes

  • NII Article ID (NAID)
    130006399290
  • NII NACSIS-CAT ID (NCID)
    AN00062446
  • Text Lang
    JPN
  • ISSN
    0021-4876
  • NDL Article ID
    028885746
  • NDL Call No.
    Z17-314
  • Data Source
    NDL  J-STAGE 
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