Quantitative Analysis of the Preferential Orientation of Collagen and Elastin Fibers in Blood Vessel with an Imaging Methodology Combined with Birefringence Measurement

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  • Ozasa Ryosuke
    Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
  • Nagaishi Takeru
    Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
  • Yamazaki Daisuke
    Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
  • Matsugaki Aira
    Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University
  • Kanzaki Machiko
    Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
  • Kuratani Toru
    Department of Minimally Invasive Cardiovascular Medicine, Osaka University Graduate School of Medicine
  • Morii Eiichi
    Department of Pathology, Osaka University Graduate School of Medicine
  • Sakata Yasushi
    Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
  • Nakano Takayoshi
    Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University

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Other Title
  • 血管中膜におけるコラーゲン／エラスチン線維配向性解明のための定量的解析手法の確立
  • ケッカン チュウマク ニ オケル コラーゲン/エラスチン センイハイコウセイ カイメイ ノ タメ ノ テイリョウテキ カイセキ シュホウ ノ カクリツ

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Abstract

<p>Blood vessel exhibits a complicated microstructure depending on the anatomical portion, which enables the realization of its proper mechanical function. In particular, tunica media plays crucial roles in mediating the structural and functional properties of blood vessels. However, the microstructural analysis with quantitative methodology has not been established. In this study, the quantitative analysis of collagen fibers and elastin fibers in tunica media was achieved by combination of a selective staining technique using fluorescent dyes and birefringence measurement with polarized microscopy. The present method quantitatively unveiled the microstructural difference between thoracic aorta and caudal artery. Both types of vessels were comprised of collagen fibers and elastin fibers running along the circumferential direction of tunica media. Interestingly, the degree of preferential orientation of these fibers was markedly increased in thoracic aorta, suggesting that the anisotropic microstructure of vessel varies depending on the anatomical site.</p>

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