Ubiquitin-proteasome system modulates zygotic genome activation in early mouse embryos and influences full-term development

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Author(s)

    • HIGUCHI Chika HIGUCHI Chika
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan
    • SHIMIZU Natsumi HOSOI Yoshihiko
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan
    • SHIN Seung-Wook MIYAMOTO Kei
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan
    • MORITA Kohtaro MATSUMOTO Kazuya
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan
    • ANZAI Masayuki SHIN Seung-Wook
    • Laboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Maryland 20892, USA
    • KATO Hiromi MORITA Kohtaro
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan
    • MITANI Tasuku NAGAI Kouhei
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan
    • YAMAGATA Kazuo ANZAI Masayuki
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan|Institute of Advanced Technology, Kindai University, Wakayama 642-0017, Japan
    • HOSOI Yoshihiko KATO Hiromi
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan|Institute of Advanced Technology, Kindai University, Wakayama 642-0017, Japan
    • MIYAMOTO Kei MITANI Tasuku
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan|Institute of Advanced Technology, Kindai University, Wakayama 642-0017, Japan
    • MATSUMOTO Kazuya YAMAGATA Kazuo
    • Laboratory of Molecular Developmental Biology, Graduate School of Biology-Oriented Science and Technology, Kindai University, Wakayama 649-6493, Japan

Abstract

<p> Maternal RNA/protein degradation and zygotic genome activation (ZGA), occurring during maternal-to-zygotic transition (MZT), are the first essential events for the development of pre-implantation embryos. Previously, we have shown the importance of the ubiquitin-proteasome system (UPS) for initiation of minor ZGA at the 1-cell stage of mouse embryos. However, little is known about the mechanism of involvement of the UPS-degraded maternal proteins in ZGA. In this study, we investigated the effect of inhibiting maternal protein degradation by the reversible proteasome inhibitor, MG132, on post-implantation development and ZGA regulation during early cleavage stages. Our study revealed that zygotic transcription by RNA polymerase II (Pol II) at the 1-cell stage was delayed and the full-term development was affected by transient proteasome inhibition during 1 to 9 h post-insemination (hpi). Furthermore, we found that the transient inhibition of proteasome activity at the 2-cell stage delayed the onset of transcription of some major ZGA genes. These results support the model hypothesizing the requirement of sequential degradation of maternal proteins by UPS for the proper onset of ZGA and normal progression of MZT in early mouse embryos.</p>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 64(1), 65-74, 2018

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

Codes

  • NII Article ID (NAID)
    130006405009
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • ISSN
    0916-8818
  • NDL Article ID
    028848918
  • NDL Call No.
    Z54-H305
  • Data Source
    NDL  J-STAGE 
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