Identification of Functional Food Factors as β₂-Adrenergic Receptor Agonists and Their Potential Roles in Skeletal Muscle

• CHIKAZAWA Miho

  Food Biochemistry Laboratory, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo

• SATO Ryuichiro

  Food Biochemistry Laboratory, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo Nutri-Life Science Laboratory, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo AMED-CREST, Japan Agency for Medical Research and Development

Bibliographic Information

Other Title

• Identification of Functional Food Factors as β₂-Adrenergic Receptor Agonists and Their Potential Roles in Skeletal Muscle

Abstract

Maintaining skeletal muscle functions by controlling muscle metabolism is of utmost importance. β₂-Adrenergic receptor (β₂-AR), which is expressed in skeletal muscle, is a member of the G-protein-coupled receptor family that plays a critical role in the maintenance of muscle mass. In the present study, using luciferase reporter assays in β₂-AR-expressing HEK293 cells, we discovered several food factors that exhibited agonistic activity at mouse or human β₂-AR. Osthole, gramine, and hordenine were identified as both mouse and human β₂-AR agonists, whereas berberine was identified as a mouse β₂-AR agonist only. Additionally, intramuscular injection of gramine or hordenine in mice facilitated gene expression of several cAMP response element binding protein targets, which is thought to result in increased skeletal muscle protein synthesis. This study provides evidence that several food factors might exert potential health effects on skeletal muscle by enhancing cAMP signaling through the activation of β₂-AR.

Journal

• Journal of Nutritional Science and Vitaminology

  Journal of Nutritional Science and Vitaminology 64 (1), 68-74, 2018

  Center for Academic Publications Japan

Keywords

• skeletal muscle
• beta 2-adrenergic receptor
• luciferase assay
• agonist
• cAMP

Details

• CRID: 1390001206323952384
• NII Article ID: 130006405194
• NII Book ID: AA00703822
• DOI: 10.3177/jnsv.64.68
• ISSN: 18817742
• ISSN: 03014800
• NDL BIB ID: 029130285
• PubMed: 29491275
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029130285
• Web Site: https://www.jstage.jst.go.jp/article/jnsv/64/1/64_68/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018288766
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed