Expression profiles of the antioxidant enzymes gene（SOD1, CAT, GPX, and HMOX1）in the skin of UV-irradiated and obese mice

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  • Nishijima Yoshimi
    Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo Department of Anatomy, Teikyo University School of Medicine
  • Ibuki Ai
    Department of Biological Science and Nursing, Graduate School of Medicine, Yokohama City University
  • Minematsu Takeo
    Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
  • Sanada Hiromi
    Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo

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  • UV照射マウスおよび肥満マウス皮膚における抗酸化酵素遺伝子（SOD1, CAT, GPX, HMOX1）の発現プロファイル

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Abstract

 Oxidative stress is an established risk factor for several dermatological diseases in obese skin. We previously reported that elevated mRNA expression of an antioxidant enzyme gene, heme oxygenase 1（HMOX1）, in the follicular cells of body hair was associated with dermal structural changes in obese subjects. Although this method enables non-invasive assessment of oxidative stress in the skin, it is necessary to evaluate the expression of antioxidant enzymes under different conditions. The purpose of this study was to measure the expression of antioxidant enzymes in skin with acutely induced and chronic elevation of oxidative stress. Using the dorsal skin of untreated wild-type mice（control）, UV-irradiated wild-type mice, and obese（ob/ob）（mice, mRNA and protein levels of four antioxidant enzymes, superoxide dismutase 1（SOD1）, catalase（CAT）, glutathione peroxidase（GPX）, and HMOX1, were evaluated. SOD1 was elevated in both UV-irradiated and obese mice, while CAT, GPX, and HMOX1 were only increased in obese mice. These differences were more prominent at the mRNA level. Thus, analysis of mRNA expression of antioxidant enzymes（SOD1, CAT, GPX, and HMOX1）skin oxidative stress.

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