UV照射マウスおよび肥満マウス皮膚における抗酸化酵素遺伝子(SOD1, CAT, GPX, HMOX1)の発現プロファイル  [in Japanese] Expression profiles of the antioxidant enzymes gene(SOD1, CAT, GPX, and HMOX1)in the skin of UV-irradiated and obese mice  [in Japanese]

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Author(s)

    • 西島 良美 Nishijima Yoshimi
    • 東京大学大学院医学系研究科健康科学・看護学専攻老年看護学/創傷看護学|帝京大学医学部解剖学講座 Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo|Department of Anatomy, Teikyo University School of Medicine
    • 伊吹 愛 Ibuki Ai
    • 横浜市立大学大学院医学系研究科・医学部看護生命科学 Department of Biological Science and Nursing, Graduate School of Medicine, Yokohama City University
    • 峰松 健夫 Minematsu Takeo
    • 東京大学大学院医学系研究科健康科学・看護学専攻老年看護学/創傷看護学 Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo
    • 真田 弘美 Sanada Hiromi
    • 東京大学大学院医学系研究科健康科学・看護学専攻老年看護学/創傷看護学 Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo

Abstract

 皮膚での酸化ストレス上昇は種々の皮膚疾患に繋がる.私達は,毛根を試料として用いたRT-PCR法による還元酵素<I>(HMOX1)</I>の発現解析が皮膚の酸化ストレスレベルを反映することを報告した.この方法は簡便かつ低侵襲な方法として有用と考えられるが,臨床応用に向けて,異なる酸化ストレスにおける抗酸化酵素の発現プロファイルを明らかにする必要がある.そこで本研究では,異なる酸化ストレスモデルマウス(UV照射群および肥満群)における抗酸化酵素SOD1,CAT,GPXおよびHMOX1の発現量を対照群と比較し,その発現プロファイルの比較検討を行った.SOD1は両群で対照群よりも高い発現量を認めた.一方CAT,GPXおよびHMOX1は肥満群でのみ上昇しており,これらの結果は特にmRNA発現解析で認められた.これにより皮膚における抗酸化酵素遺伝子(SOD1, CAT, GPX, HMOX1)のmRNA発現解析は,酸化ストレスの原因推定も可能な簡便かつ低侵襲的な評価法として皮膚疾患の予防的スキンケアへと寄与できる可能性が考えられた.

 Oxidative stress is an established risk factor for several dermatological diseases in obese skin. We previously reported that elevated mRNA expression of an antioxidant enzyme gene, heme oxygenase 1<I>(HMOX1)</I>, in the follicular cells of body hair was associated with dermal structural changes in obese subjects. Although this method enables non-invasive assessment of oxidative stress in the skin, it is necessary to evaluate the expression of antioxidant enzymes under different conditions. The purpose of this study was to measure the expression of antioxidant enzymes in skin with acutely induced and chronic elevation of oxidative stress. Using the dorsal skin of untreated wild-type mice(control), UV-irradiated wild-type mice, and obese<I>(ob/ob)</I>(mice, mRNA and protein levels of four antioxidant enzymes, superoxide dismutase 1(SOD1), catalase(CAT), glutathione peroxidase(GPX), and HMOX1, were evaluated. SOD1 was elevated in both UV-irradiated and obese mice, while CAT, GPX, and HMOX1 were only increased in obese mice. These differences were more prominent at the mRNA level. Thus, analysis of mRNA expression of antioxidant enzymes(SOD1, CAT, GPX, and HMOX1)skin oxidative stress.

Journal

  • Journal of Nursing Science and Engineering

    Journal of Nursing Science and Engineering 3(1), 13-20, 2016

    The Society for Nursing Science and Engineering

Codes

  • NII Article ID (NAID)
    130006406635
  • Text Lang
    JPN
  • ISSN
    2188-4323
  • Data Source
    J-STAGE 
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