BNIP3 expression in bovine follicle and corpus luteum

• NISHIMURA Ryo

  Laboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan

• OKUDA Kiyoshi

  Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan

• GUNJI Yosuke

  Laboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan United Graduate School of Veterinary Science, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8511, Japan

• KHALID Ahmed Magzoub

  United Graduate School of Veterinary Science, Yamaguchi University, Yoshida 1677-1, Yamaguchi 753-8511, Japan Laboratory of Veterinary Biochemistry, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan Department of Genetics and Animal Breeding, Faculty of Animal Production, University of Khartoum, Shambat 13314, Sudan

• YAMANO Yoshiaki

  Laboratory of Veterinary Biochemistry, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan

• YAMASHITA Yasuhisa

  Laboratory of Animal Physiology, Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, 562 Nanatuka-cho, Shobara, Hiroshima 727-0023, Japan

• HISHINUMA Mitsugu

  Laboratory of Theriogenology, Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori 680-8553, Japan

Abstract

BNIP3 (BCL2/adenovirus E1B nineteen kilodalton interacting protein-3), a member of the BCL2 family, is activated under hypoxic conditions and induces apoptosis or mitochondrial autophagy for adapting cells to hypoxia. The physiological roles of BNIP3 in the mammalian ovary are still unclear. In order to understand the role of BNIP3 in the bovine ovary, we examined its mRNA and protein expressions of BNIP3 in follicular granulosa cells and corpus luteum (CL). BNIP3 mRNA and protein expressions in granulosa cells from large follicles (>10 mm) at the follicular stage were much higher than those in small follicles (2–8 mm). BNIP3 mRNA and protein expressions in the CL peaked at the early luteal stage. In bovine granulosa cells cultured for 6 hr under hypoxia (3% O₂) and normoxia (20% O₂), BNIP3 mRNA expression was higher under hypoxia. These results of the present study suggest that BNIP3 has some roles in luteal formation in the bovine ovary, and that the highly expressed BNIP3 in the granulosa cells from large follicles at the follicular stage is related to the roles of BNIP3 in the luteal formation.

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 80 (2), 368-374, 2018

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• bovine
• granulosa cell
• hypoxia
• luteal cell
• ovary

Details

• CRID: 1390282681404707712
• NII Article ID: 130006410764
• DOI: 10.1292/jvms.17-0267
• ISSN: 13477439
• ISSN: 09167250
• PubMed: 29269703
• Web Site: https://www.jstage.jst.go.jp/article/jvms/80/2/80_17-0267/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed