<b>Effect of Prostaglandin E<sub>2 </sub>on Human Dental Follicle Cells during Osteogenic </b><b>Differentiation </b>

DOI Web Site 25 References
  • Yoshimoto Hidesuke
    Nihon University Graduate School of Dentistry at Matsudo, Maxillofacial Surgery, Matsudo, Chiba 271-8587, Japan
  • Ogura Naomi
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

Search this article

Abstract

<p>　Stem/progenitor cells isolated from human dental follicles differentiate into osteogenic cells. To investigate factors associated with osteogenic differentiation/mineralization in human dental follicle cells (hDFCs), we performed gene expression profiling of hDFCs during osteogenic differentiation. Cyclooxygenase (COX)-1 and -2 were up-regulated in hDFCs cultured in osteogenic induction medium(OIM)compared to growth medium (GM). Prostaglandin E2 (PGE2), which is the most studied prostanoid derived from arachidonic acid through the actions of COXs, may regulate bone metabolism. All PGE2 E-type prostanoid receptors (EP), EP1-EP4, were expressed in hDFCs. Real-time PCR showed that the expression of EP2 and EP4 was increased in hDFCs cultured in OIM and GM at days 10 and 17 compared to day 0. We investigated the action of PGE2 in osteogenic differentiation using hDFCs. PGE2 decreased gene expression levels of osterix and alkaline phosphatase, which are factors associated with osteogenic differentiation. PGE2 elicited inhibitory action on matrix mineralization of hDFCs as seen with alizarin red S staining. These findings suggest that PGE2 may inhibit osteogenic differentiation/mineralization of stem/progenitor cells.</p>

Journal

References(25)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top