Effect of collagenase concentration on the isolation of small adipocytes from human buccal fat pad
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- Tsurumachi Niina
- Department of Orthodontics, Nihon University School of Dentistry
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- Akita Daisuke
- Department of Partial Denture Prosthodontics, Nihon University School of Dentistry
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- Kano Koichiro
- Laboratory of Cell and Tissue Biology, College of Bioresource Sciences, Nihon University
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- Matsumoto Taro
- Department of Functional Morphology Division of Cell Regeneration and Transplantation, Nihon University School of Medicine
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- Toriumi Taku
- Department of Anatomy, Nihon University School of Dentistry
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- Kazama Tomohiko
- Department of Functional Morphology Division of Cell Regeneration and Transplantation, Nihon University School of Medicine
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- Oki Yoshinao
- Laboratory of Cell and Tissue Biology, College of Bioresource Sciences, Nihon University
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- Saito-Tamura Yoko
- Department of Orthodontics, Nihon University School of Dentistry
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- Tonogi Morio
- Department of Oral and Maxillofacial Surgery, Nihon University School of Dentistry
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- Shimizu Noriyoshi
- Department of Orthodontics, Nihon University School of Dentistry
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- Honda Masaki
- Department of Oral Anatomy, Aichi Gakuin University School of Dentistry
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<p>Dedifferentiated fat (DFAT) cells were isolated from mature adipocytes using the ceiling culture method. Recently, we successfully isolated DFAT cells from adipocytes with a relatively small size (<40 μm). DFAT cells have a higher osteogenic potential than that of medium adipocytes. Therefore, the objective of this study was to determine the optimal concentration of collagenase solution for isolating small adipocytes from human buccal fat pads (BFPs). Four concentrations of collagenase solution (0.01%, 0.02%, 0.1%, and 0.5%) were used, and their effectiveness was assessed by the number of small adipocytes and DFAT cells isolated. The total number of floating adipocytes that dissociated with 0.02% collagenase was 2.5 times of that dissociated with 0.1% collagenase. The number of floating adipocytes with a diameter of ≤29 μm that dissociated with 0.02% collagenase was thrice of those dissociated with 0.1% and 0.5% collagenase. The number of DFAT cells that dissociated with 0.02% collagenase was 1.5 times of that dissociated with 0.1% collagenase. In addition, DFAT cells that dissociated with 0.02% collagenase had a higher osteogenic differentiation potential than those that dissociated with 0.1% collagenase. These results suggest that 0.02% is the optimal collagenase concentration for isolating small adipocytes from BFPs.</p>
収録刊行物
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- Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ
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Ｊｏｕｒｎａｌ ｏｆ Ｏｒａｌ Ｓｃｉｅｎｃｅ 60 (1), 14-23, 2018
日本大学歯学部
