Nitrative DNA damage in cultured macrophages exposed to indium oxide
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- Afroz Tahmina
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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- Hiraku Yusuke
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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- Ma Ning
- Faculty of Nursing, Suzuka University of Medical Science
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- Ahmed Sharif
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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- Oikawa Shinji
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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- Kawanishi Shosuke
- Faculty of Pharmaceutical Sciences, Suzuka University of Medical Science
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- Murata Mariko
- Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine
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Abstract
<p>Objectives: Indium compounds are used in manufacturing displays of mobile phones and televisions. However, these materials cause interstitial pneumonia in exposed workers. Animal experiments demonstrated that indium compounds caused lung cancer. Chronic inflammation is considered to play a role in lung carcinogenesis and fibrosis induced by particulate matters. 8-Nitroguanine (8-nitroG) is a mutagenic DNA lesion formed during inflammation and may participate in carcinogenesis. To clarify the mechanism of carcinogenesis, we examined 8-nitroG formation in indium-exposed cultured cells. Methods: We treated RAW 264.7 mouse macrophages with indium oxide (In2O3) nanoparticles (primary diameter: 30-50 nm), and performed fluorescent immunocytochemistry to detect 8-nitroG. The extent of 8-nitroG formation was evaluated by quantitative image analysis. We measured the amount of nitric oxide (NO) in the culture supernatant of In2O3-treated cells by the Griess method. We also examined the effects of inhibitors of inducible NO synthase (iNOS) and endocytosis on In2O3-induced 8-nitroG formation. Results: In2O3 significantly increased the intensity of 8-nitroG formation in RAW 264.7 cells in a dose-dependent manner. In2O3-induced 8-nitroG formation was observed at 2 h and further increased at 4 h, and the amount of NO released from In2O3-exposed cells was significantly increased at 2-4 h compared with the control. 8-NitroG formation was suppressed by 1400W (an iNOS inhibitor), methyl-β-cyclodextrin and monodansylcadaverine (inhibitors of caveolae- and clathrin-mediated endocytosis, respectively). Conclusions: These results suggest that endocytosis and NO generation participate in indium-induced 8-nitroG formation. NO released from indium-exposed inflammatory cells may induce DNA damage in adjacent lung epithelial cells and contribute to carcinogenesis.</p>
Journal
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- Journal of Occupational Health
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Journal of Occupational Health 60 (2), 148-155, 2018
Japan Society for Occupational Health
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Details 詳細情報について
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- CRID
- 1390001204456276864
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- NII Article ID
- 130006565471
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- NII Book ID
- AA11090645
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- ISSN
- 13489585
- 13419145
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- NDL BIB ID
- 028896377
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- PubMed
- 29187674
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed