Bacterial glycerol oxidation coupled to sulfate reduction at neutral and acidic pH
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- Santos Sidnei C.
- Laboratory of Microbiology, Wageningen University Studies Institute in Health and Biological, Federal University of South and Southeast of Pará
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- Liebensteiner Martin G.
- Laboratory of Microbiology, Wageningen University
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- van Gelder Antonie H.
- Laboratory of Microbiology, Wageningen University
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- Dimitrov Mauricio R.
- Laboratory of Microbiology, Wageningen University
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- Almeida Paulo F.
- Laboratory of Biotechnology and Ecology of Microorganisms, Department of Bio-Interaction Sciences, Federal University of Bahia
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- Quintella Cristina M.
- Laboratory of Kinetic and Molecular Dynamic, Institute of Chemistry, Federal University of Bahia
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- Stams Alfons J. M.
- Laboratory of Microbiology, Wageningen University Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho
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- Sánchez-Andrea Irene
- Laboratory of Microbiology, Wageningen University
抄録
<p>Glycerol is a main co-product of biodiesel production. Crude glycerol may serve as a cheap and attractive substrate in biotechnological applications, e.g. for the production of valuable chemicals or as an electron donor for reduction processes. In this work, sulfate reduction with glycerol was studied at neutral and acidic pH using bioreactor sludge samples and Tinto River sediments as a source of inoculum, respectively. Communities of sulfate-reducing bacteria (SRB) and fermentative bacteria were co-enriched at both pH values. Molecular analyses revealed that sequences belonging to Desulfomicrobium genus were dominant in the cultures enriched at pH 7, while Desulfosporosinus sequences dominated in the culture enriched at pH 4. Glycerol conversion was coupled to sulfate reduction, but the substrate was incompletely oxidized to acetate in the neutrophilic enrichments, and acetate, lactate, and 1,3-propanediol under low pH conditions. Two strains belonging to Desulfomicrobium and Proteiniphilum genera were isolated from the neutrophilic enrichments, but the first isolate was not able to use glycerol, which suggests a syntrophic relationship between glycerol-degrading fermentative bacteria and SRB. A Clostridium strain able to grow with glycerol was isolated from the low pH enrichment. Our data indicate that glycerol promotes the growth of sulfate-reducing communities to form sulfide, which can be used to precipitate and recover heavy metals.</p>
収録刊行物
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- The Journal of General and Applied Microbiology
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The Journal of General and Applied Microbiology 64 (1), 1-8, 2018
公益財団法人 応用微生物学・分子細胞生物学研究奨励会