Hypophosphatemia occurs with insulin administration during refeeding by total parenteral nutrition in rats

• Kawamura Hiromi

  Graduate school of Human Science and Environment

• Tanaka Sarasa

  School of Human Science and Environment, University of Hyogo

• Uenami Yuri

  School of Human Science and Environment, University of Hyogo

• Tani Mariko

  Graduate school of Human Science and Environment

• Ishitani Midori

  Graduate school of Human Science and Environment

• Morii Saeko

  School of Human Science and Environment, University of Hyogo

• Sakaue Motoyoshi

  Graduate school of Human Science and Environment

• Ito Mikiko

  Graduate school of Human Science and Environment

Abstract

Refeeding syndrome (RFS) is characterized by the metabolic and clinical changes that occur following aggressive nutritional supplementation in malnourished patients. Hypophosphatemia is the hallmark of RFS and is key to its prevention and treatment in clinical practice. However, the mechanism of hypophosphatemia during RFS is unclear because of the lack of an animal model. In this study, we developed a rat RFS model as a first step to clarifying the molecular mechanism. After establishing the parenteral route, rats were fasted for 5 days and refeeding was started using total parenteral nutrition. The animals were infused with a high calorie solution with or without insulin administration. Results showed that plasma phosphate levels did not decrease in rats infused with the high calorie solution alone;in contrast, a 20% reduction compared to baseline was observed in rats administered insulin. In addition, rats infused with the high calorie solution containing added phosphate did not present with hypophosphatemia. Thus, we developed a rat RFS model with hypophosphatemia by tube feeding and insulin administration, and demonstrated the importance of phosphate in preventing refeeding hypophosphatemia. J. Med. Invest. 65:50‐55, February, 2018

Journal

• The Journal of Medical Investigation

  The Journal of Medical Investigation 65 (1.2), 50-55, 2018

  The University of Tokushima Faculty of Medicine

Keywords

• Refeeding syndrome
• hypophosphatemia
• total parenteral nutrition
• animal model
• insulin

Details

• CRID: 1390282679224614528
• NII Article ID: 130006575588
• NII Book ID: AA11166929
• DOI: 10.2152/jmi.65.50
• ISSN: 13496867
• ISSN: 13431420
• PubMed: 29593193
• Web Site: https://repo.lib.tokushima-u.ac.jp/111409
• Web Site: https://www.jstage.jst.go.jp/article/jmi/65/1.2/65_50/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2019170634
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed