抗非定型Aeromonas salmonicidaモノクローナル抗体のニシキゴイの“新穴あき病”診断への利用

• Somamoto Tomonori

  Laboratory of Marine Biochemistry, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Maruyama Shinpei

  Laboratory of Marine Biochemistry, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Nagasawa Takahiro

  Laboratory of Marine Biochemistry, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Nakao Miki

  Laboratory of Marine Biochemistry, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University

• Sato Atsushi

  Kyorin Co. Ltd.

• Hatta Hajime

  Department of Food Nutrition, Kyoto Women’s University

• Sato Mitsuru

  Animal Bioregulation Unit, Division of Animal Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization

• Murakami-Yamaguchi Yukie

  Kyoto College of Nutritional & Medical Sciences

• Kizu-Mori Kumiko

  Kyoto College of Nutritional & Medical Sciences

• Hirakawa Yuki

  Department of Food Nutrition, Kyoto Women’s University

• Narita Hiroshi

  Department of Food Nutrition, Kyoto Women’s University

書誌事項

タイトル別名

• Development of Anti-atypical <i>Aeromonas salmonicida</i> Monoclonal Antibodies for Diagnosis of “New Ulcer Disease” in Koi Carp
• Development of Anti-atypical Aeromonas salmonicida Monoclonal Antibodies for Diagnosis of "New Ulcer Disease" in Koi Carp

抄録

To develop an accurate diagnosis of “new ulcer disease” in koi carp, we produced four monoclonal antibodies (mAbs) against a strain of atypical Aeromonas salmonicida isolated from koi carp. These mAbs did not cross-react with an isolate of atypical A. salmonicida from Japanese flounder and other pathogenic bacteria. Re-isolation from artificially infected koi carp was achieved by selecting the blue colonies on agar medium containing Coomassie brilliant blue, and some of the colonies were detected by immunofluorescent staining using the mAbs. These results suggested that the mAbs can distinguish atypical A. salmonicida from koi carp from resident aeromonads.

収録刊行物

• 魚病研究

  魚病研究 53 (1), 36-39, 2018

  日本魚病学会

キーワード

• atypical <i>Aeromonas salmonicida</i>
• monoclonal antibody
• “new ulcer disease”
• koi carp

詳細情報

• CRID: 1390282681436958336
• NII論文ID: 130006619196
• NII論文ID: 220000156862
• NII書誌ID: AN00063165
• DOI: 10.3147/jsfp.53.36
• ISSN: 18817335
• ISSN: 0388788X
• HANDLE: 2324/4784368
• NDL書誌ID: 028943851
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030921439
• Web Site: http://id.ndl.go.jp/bib/028943851
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028943851
• Web Site: https://www.jstage.jst.go.jp/article/jsfp/53/1/53_36/_pdf
• 本文言語コード: en
• データソース種別:
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