Prevention of Evaporation Caused by a Rapid Immunohistochemistry Method Applying Non-contact Alternating-Current Electric-Field Mixing
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- HOSHINO Iku
- 秋田大学医学部 胸部外科
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- IMAI Kazuhiro
- 秋田大学医学部 胸部外科
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- NAKAMURA Ryuta
- 秋田県産業技術センター
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- SAITO Yoshitaro
- 秋田大学医学部 胸部外科
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- FUJISHIMA Satoshi
- 秋田大学医学部 胸部外科
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- KURIHARA Nobuyasu
- 秋田大学医学部 胸部外科
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- Wakamatsu Yuki
- 秋田大学医学部 胸部外科
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- SAITO Hajime
- 秋田大学医学部 胸部外科
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- TERATA Kaori
- 秋田大学医学部 胸部外科
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- SATO Yusuke
- 秋田大学医学部 胸部外科
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- MOTOYAMA Satoru
- 秋田大学医学部 胸部外科
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- NANJO Hiroshi
- 秋田大学医学部附属病院 病理診断科/病理部
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- AKAGAMI Yoichi
- 秋田県産業技術センター
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- MINAMIYA Yoshihiro
- 秋田大学医学部 胸部外科
Bibliographic Information
- Other Title
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- 電界非接触撹拌技術を応用した迅速免疫組織化学染色装置における液滴蒸散予防の工夫
Abstract
<p>It is widely recognized that pathology is the most important factor for staging and selecting effective chemotherapy for patients with cancer. Immunohistochemistry (IHC) is a reliable screening method, but intra-operative diagnosis by frozen section with IHC is not possible because IHC takes approximately 6 hours. We recently developed a rapid-IHC method that makes use of an alternating current (AC) electric field to facilitate the antigen-antibody reaction, and reported its usefulness for detection of lung cancer and breast cancer. The antibody is mixed within microdroplets as the voltage is switched on and off at specific intervals. The resultant Coulomb force stirs the diluted solution on the sections, which increases the opportunity for contact. The rapid-IHC device reduces the time required for IHC as well as the amount of antibody required for analyses. The device may also be applied for in situ hybridization (ISH); however, ISH requires a warm temperature, such as 37℃, and a longer processing time than the rapid-IHC method. Consequently, evaporation can be a problem during ISH. The aim of the present study was to evaluate the utility and reliability of an oil cover and cover cap made of polyethylene terephthalate (PET) for preventing evaporation due to the AC electric field applied to facilitate the antigen-antibody reaction. We have shown that both the oil cover and PET cover cap prevented evaporation when using the rapid-IHC device.</p>
Journal
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- Journal of the Japan Society for Precision Engineering
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Journal of the Japan Society for Precision Engineering 84 (4), 383-387, 2018
The Japan Society for Precision Engineering
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Keywords
Details 詳細情報について
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- CRID
- 1390282679807107840
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- NII Article ID
- 130006638790
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- ISSN
- 1882675X
- 09120289
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed