Evaluating the electrical impedance and mucus-related gene expression of uterine endometrial tissues in mares

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Author(s)

    • KIKUCHI Kohta KIKUCHI Kohta
    • Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan
    • KOZAI Keisuke KOZAI Keisuke
    • Laboratory of Animal Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan|Present: Institute for Reproductive Health and Regenerative Medicine, Departments of Pathology and Laboratory Medicine and Pediatrics, University of Kansas Medical Center, Kansas City, KS, USA
    • HOJO Takuo HOJO Takuo
    • Livestock and Grassland Research Division, Kyushu Okinawa Agricultural Research Center, NARO, Kumamoto 861-1192, Japan
    • SAKATANI Miki SAKATANI Miki
    • Livestock and Grassland Research Division, Kyushu Okinawa Agricultural Research Center, NARO, Kumamoto 861-1192, Japan
    • OKUDA Kiyoshi OKUDA Kiyoshi
    • Laboratory of Animal Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan|Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan
    • BAI Hanako BAI Hanako
    • Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan
    • KAWAHARA Manabu KAWAHARA Manabu
    • Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan
    • TAKAHASHI Masashi TAKAHASHI Masashi
    • Laboratory of Animal Genetics and Reproduction, Department of Animal Science, Research Faculty of Agriculture, Hokkaido University, Hokkaido 060-8589, Japan

Abstract

<p> We investigated the electrical impedance of the reproductive tracts (vagina and uterine endometrial tissues) and the expression of mucus-related genes to identify the stage of the estrous cycle in mares. We first examined vaginal impedance in native Hokkaido mares during their estrous cycle and found no significant differences. However, impedance levels tended to decrease towards ovulation. Furthermore, we investigated the estrous cycle by measuring the electrical impedance of the uterine endometrial tissues obtained from carcasses of mares. We found that impedance levels in the endometrial tissues decreased in the regressed phase of the corpus luteum (CL). Expression of mucus-related genes (<i>ATP1A1</i>, <i>CFTR</i>, <i>AQP3</i>, and <i>AQP5</i>) varied at different stages of the estrous cycle. Among them, <i>AQP3</i> expression was consistent with previous reports. We concluded that electrical impedance in the uterine endometrial tissues of mares could be potentially used to verify the presence of active CL in horses for experimental purposes. However, further studies are needed to determine the reference value and to identify the day of the estrous cycle in mares.</p>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 64(2), 193-197, 2018

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

Codes

  • NII Article ID (NAID)
    130006699687
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0916-8818
  • NDL Article ID
    028937317
  • NDL Call No.
    Z54-H305
  • Data Source
    NDL  IR  J-STAGE 
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