Identification of quantitative trait loci associated with the susceptibility of mouse spermatozoa to cryopreservation

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Author(s)

    • LIU Jinsha LIU Jinsha
    • RIKEN BioResource Center, Ibaraki 305-0074, Japan|Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8572, Japan
    • INOUE Kimiko INOUE Kimiko
    • RIKEN BioResource Center, Ibaraki 305-0074, Japan|Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8572, Japan
    • OGURA Atsuo OGURA Atsuo
    • RIKEN BioResource Center, Ibaraki 305-0074, Japan|Graduate School of Life and Environmental Science, University of Tsukuba, Ibaraki 305-8572, Japan|The Center for Disease Biology and Integrative Medicine, Faculty of Medicine, University of Tokyo, Tokyo 113-0033, Japan

Abstract

<p> Although it is known that the susceptibility of mouse spermatozoa to freezing-thawing varies greatly with genetic background, the underlying mechanisms remain to be elucidated. In this study, to map genetic regions responsible for the susceptibility of spermatozoa to freezing-thawing, we performed <i>in vitro</i> fertilization using spermatozoa from recombinant inbred mice derived from the C57BL/6J and DBA/2J strains, whose spermatozoa showed distinct fertilization abilities after freezing. Genome-wide interval mapping identified two suggestive quantitative trait loci (QTL) associated with fertilization on chromosomes 1 and 11. The strongest QTL on chromosome 11 included 70 genes at 59.237260–61.324742 Mb and another QTL on chromosome 1 included 43 genes at 153.969506–158.217850 Mb. These regions included at least 15 genes involved with testicular expression and possibly with capacitation or sperm motility. Specifically, the <i>Abl2</i> gene on chromosome 1, which may affect subcellular actin distribution, had polymorphisms between C57BL/6J and DBA/2J that caused at least three amino acid substitutions. A correlation analysis using recombinant inbred strains revealed that the fertilization rate was strongly correlated with the capacitation rate of frozen-thawed spermatozoa after preincubation. This result is consistent with the fact that C57BL/6J frozen-thawed spermatozoa recover their fertilization capacity following treatment with methyl-β-cyclodextrin to enhance sperm capacitation. Thus, our data provide important clues to the molecular mechanisms underlying cryodamage to mouse spermatozoa.</p>

Journal

  • Journal of Reproduction and Development

    Journal of Reproduction and Development 64(2), 117-127, 2018

    THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT

Codes

  • NII Article ID (NAID)
    130006699699
  • NII NACSIS-CAT ID (NCID)
    AA10936678
  • Text Lang
    ENG
  • ISSN
    0916-8818
  • NDL Article ID
    028937216
  • NDL Call No.
    Z54-H305
  • Data Source
    NDL  J-STAGE 
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