Protective Effect of Group 3 LEA Model Peptides on the Thermal Denaturation of Proteins

• FURUKI Takao

• SAKURAI Minoru

Bibliographic Information

Other Title

• グループ3 LEA タンパク質を模倣した機能ペプチドによる タンパク質の熱変性抑制
• グループ 3LEA タンパクシツ オ モホウ シタ キノウ ペプチド ニ ヨル タンパクシツ ノ ネツ ヘンセイ ヨクセイ

Abstract

We tested the protective effect of the following reagents on the thermal denaturation of lysozyme: 1) PvLEA-22, which consists of two tandem repeats of the 11-mer motif characteristic to LEA proteins from an African sleeping chironomid, 2) its scrambled-type peptide, which has the amino acid composition identical with that of PvLEA-22, although its sequence is scrambled, and 3) a disaccharide, trehalose. The results of turbidity, enzyme activity and circular dichroism (CD) spectrum measurements showed that the LEA model peptides can inhibit the thermal denaturation of lysozyme.

Journal

• Cryobiology and Cryotechnology

  Cryobiology and Cryotechnology 63 (2), 139-142, 2017

  Japanese Society of Cryobiology and Cryotechnology

Keywords

• Group3 LEA model peptide
• Thermal denaturation
• Lysozyme
• Cross protection
• グループ3LEAモデルペプチド
• 熱変性
• リゾチーム
• 交差耐性効果

Details

• CRID: 1390282680063476480
• NII Article ID: 130006706896
• NII Book ID: AN10448734
• DOI: 10.20585/cryobolcryotechnol.63.2_139
• ISSN: 24241555
• ISSN: 13407902
• NDL BIB ID: 028708578
• Web Site: http://id.ndl.go.jp/bib/028708578
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028708578
• Web Site: https://search.jamas.or.jp/link/ui/2018271505
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed