Identification of a Novel Mutant <i>pAMT</i> Allele Responsible for Low-pungency and Capsinoid Production in Chili Pepper: Accession 'No. 4034' (<i>Capsicum chinense</i>)

Access this Article

Search this Article

Author(s)

Abstract

<p>Capsinoids are low-pungent capsaicinoid analogues in chili pepper fruits. They exhibit various bioactivities in humans similar to capsaicinoids, but do not produce a nasty burning sensation, encouraging their application in foods and supplements. Previous reports demonstrated that loss-of-function of <i>putative aminotransferase</i> (<i>pAMT</i>) leads to low-pungency and capsinoid accumulation. Therefore, the <i>pamt</i> allele is a useful gene in chili pepper breeding programs to enhance health-promoting properties. Eight loss-of-function alleles have been identified in the <i>Capsicum</i> genus, but the variation in <i>pamt</i> alleles remains to be fully elucidated. In this study, we identified one novel loss-of-function allele from the analysis of low-pungent chili pepper 'No. 4034' (<i>C. chinense</i>). 'No. 4034' contained mainly capsinoid with an undetectable level of capsaicinoid. A genetic complementation test was conducted by crossing 'No. 4034' with other accessions. The results indicated that 'No. 4034' possessed a loss-of-function <i>pamt</i> allele. Sequence analysis showed that the novel mutant allele contained a 7-bp insertion (TCGGTAC) in the 16th exon region, which we designated as <i>pamt</i><sup>9</sup>. The insertion caused a frameshift mutation and resulted in a truncated protein. Gene expression analysis showed that the expression level of <i>pAMT</i> specifically decreased among biosynthetic genes tested here in 'No. 4034', compared with that of pungent accession. <i>pamt</i><sup>9</sup> will be useful for low-pungency and capsinoid breeding, and will provide additional information for variations in <i>pAMT</i> mutants.</p>

Journal

  • The Horticulture Journal

    The Horticulture Journal 87(2), 222-228, 2018

    The Japanese Society for Horticultural Science

Codes

  • NII Article ID (NAID)
    130006708534
  • NII NACSIS-CAT ID (NCID)
    AA12708073
  • Text Lang
    ENG
  • ISSN
    2189-0102
  • NDL Article ID
    028937247
  • NDL Call No.
    Z78-A456
  • Data Source
    NDL  J-STAGE 
Page Top