A genetic link between epigenetic repressor AS1–AS2 and DNA replication factors in establishment of adaxial–abaxial leaf polarity of <i>Arabidopsis</i>

• Luong Toan Quy

  Graduate School of Bioscience and Biotechnology, Chubu University

• Keta Sumie

  Graduate School of Bioscience and Biotechnology, Chubu University

• Asai Toshiharu

  Graduate School of Bioscience and Biotechnology, Chubu University

• Kojima Shoko

  Graduate School of Bioscience and Biotechnology, Chubu University

• Nakagawa Ayami

  Graduate School of Bioscience and Biotechnology, Chubu University

• Micol José Luis

  Instituto de Bioingeniería, Universidad Miguel Hernández

• Xia Shitou

  Hunan Provincial Key Laboratory of Phytohormones and Growth and Development, Hunan Agricultural University

• Machida Yasunori

  Division of Biological Science, Graduate School of Science, Nagoya University

• Machida Chiyoko

  Graduate School of Bioscience and Biotechnology, Chubu University

Bibliographic Information

Other Title

• A genetic link between epigenetic repressor AS1-AS2 and DNA replication factors in establishment of adaxial-abaxial leaf polarity of Arabidopsis

Abstract

Balanced development of adaxial and abaxial domains in leaf primordia is critical for the formation of flat symmetric leaf lamina. Arabidopsis ASYMMETRIC LEAVES1 (AS1) and AS2 proteins form a complex (AS1–AS2), which acts as key regulators for the adaxial development by the direct repression of expression of the abaxial gene ETTIN/AUXIN RESPONSE FACTOR3 (ETT/ARF3). Many modifier mutations have been identified, which enhance the defect of as1 and as2 mutations to generate abaxialized filamentous leaves without adaxial traits, suggesting that the development of the adaxial domain is achieved by cooperative repression by AS1–AS2 and the wild-type proteins corresponding to the modifiers. Mutations of several genes for DNA replication-related chromatin remodeling factors such as Chromatin Assembly Factor-1 (CAF-1) have been also identified as modifiers. It is still unknown, however, whether mutations in genes involved in DNA replication themselves might act as modifiers. Here we report that as1 and as2 mutants grown in the presence of hydroxyurea, a known inhibitor of DNA replication, form abaxialized filamentous leaves in a concentration-dependent manner. We further show that a mutation of the INCURVATA2 (ICU2) gene, which encodes the putative catalytic subunit of DNA polymerase α, and a mutation of the Replication Factor C Subunit3 (RFC3) gene, which encodes a protein used in replication as a clamp loader, act as modifiers. In addition, as2-1 icu2-1 double mutants showed increased mRNA levels of the genes for leaf abaxialization. These results suggest a tight link between DNA replication and the function of AS1–AS2 in the development of flat leaves.

Journal

• Plant Biotechnology

  Plant Biotechnology 35 (1), 39-49, 2018

  Japanese Society for Plant Biotechnology

Keywords

• <i>ASYMMETRIC LEAVES2</i>
• DNA replication
• <i>ICU2</i>
• leaf development
• <i>RFC3</i>

Details

• CRID: 1390282679305882240
• NII Article ID: 130006727681
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.18.0129b
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 028992563
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028992563
• Web Site: https://www.jstage.jst.go.jp/article/plantbiotechnology/35/1/35_18.0129b/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed