Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary
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- Liu Jin
- Department of Occupational and Environmental Health, University of Public Health and Key Laboratory of Environment and Health, Fujian Medical University Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences
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- Zhang Wenchang
- Department of Occupational and Environmental Health, University of Public Health and Key Laboratory of Environment and Health, Fujian Medical University
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- Wu Zhiren
- Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences
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- Dai Lei
- Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences
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- Koji Takehiko
- Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences
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Abstract
<p>For a better understanding of epigenetic regulation of cell differentiation, it is important to analyze DNA methylation at a specific site. In this study, we examined changes in the methylation level of CCGG and GATCG sites during mouse folliculogenesis in paraffin-embedded sections of mouse ovaries. For the purpose, we used a new method, histo endonuclease-linked detection of methylation sites of DNA (HELMET), designed to detect methylation sites of DNA with a specific sequence in a tissue section. Unlike the global level of DNA methylation, which was no change in immunohistochemical staining of 5-methylcytosine throughout folliculogenesis, we found that there were hypermethylation of CCGG and GATCG sites in most of the granulosa cells of tertiary follicles compared to that of primary and secondary follicles. Interestingly, TUNEL-positive granulosa cells, which were frequent in mammalian folliculogenesis, became markedly Hpa II-reactive and Sau3A I-reactive, indicating that the CCGG and GATCG sites may be preferentially demethylated during apoptosis.</p>
Journal
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- ACTA HISTOCHEMICA ET CYTOCHEMICA
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ACTA HISTOCHEMICA ET CYTOCHEMICA 51 (2), 93-100, 2018
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
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Details 詳細情報について
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- CRID
- 1390001204861490176
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- NII Article ID
- 130006727960
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- ISSN
- 13475800
- 00445991
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed