Development of Micro Inclined Well Array for Trapping Single Cells

• Tsukamoto Takuya

  Gunma University

• Furuya Naoki

  Kagawa University

• Shimagami Takuya

  Kagawa University

• Sato Rui

  Gunma University

• Shimokawa Fusao

  Kagawa University

• Akimitsu Kazuya

  Kagawa University

• Suzuki Takaaki

  Gunma University JST-PRESTO

Bibliographic Information

Other Title

• 単一細胞固定のためのマイクロ傾斜ウェルアレイの開発
• タンイツ サイボウ コテイ ノ タメ ノ マイクロ ケイシャ ウェルアレイ ノ カイハツ

Abstract

We investigated the shape of a well to trap single cells for the purpose of improving the cell trapping rate after cell seeding and liquid exchange in a cell microarray. The wells having vertical and inclined tapered side-wall were made using three-dimensional UV photolithography. Changes in cell trapping rate by the shape of the wells were evaluated by trapping tests with fluorescent microbeads and living cells. As a result of trapping test with living cells, bead residual rate of the microarray with inclined tapered wells is up to 2.9 times higher than that with vertical wells.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 138 (5), 208-213, 2018-05-01

  The Institute of Electrical Engineers of Japan

Keywords

• well array
• single cell
• 3D photolithography

Details

• CRID: 1390001204461447424
• NII Article ID: 130006729821
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.138.208
• ISSN: 13475525
• ISSN: 13418939
• NDL BIB ID: 029060486
• Web Site: http://id.ndl.go.jp/bib/029060486
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I029060486
• Web Site: https://www.jstage.jst.go.jp/article/ieejsmas/138/5/138_208/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed