Diabetes Mellitus Induces Hyperreactivity of 5-Hydroxytryptamine (5-HT)-Induced Constriction in Human Internal Thoracic Artery and Is Associated with Increase in the Membrane Protein Level of 5-HT_2A Receptor

• Shiba Tatsuo

  First Department of Pharmacology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare

• Yokota Atsuko

  Department of Cardiovascular Surgery, Miyazaki Prefectural Nobeoka Hospital Department of Surgery, Faculty of Medicine, Miyazaki University

• Gamoh Shuji

  First Department of Pharmacology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare

• Tanaka-Totoribe Naoko

  First Department of Pharmacology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare

• Kuwabara Masachika

  Kuwabara Clinic

• Nakamura Eisaku

  Department of Surgery, Faculty of Medicine, Miyazaki University

• Hayase Takahiro

  Department of Cardiovascular Surgery, Miyazaki Prefectural Nobeoka Hospital

• Nakamura Kunihide

  Department of Surgery, Faculty of Medicine, Miyazaki University

• Yamamoto Ryuichi

  First Department of Pharmacology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare

Bibliographic Information

Other Title

• Diabetes Mellitus Induces Hyperreactivity of 5-Hydroxytryptamine (5-HT)-Induced Constriction in Human Internal Thoracic Artery and Is Associated with Increase in the Membrane Protein Level of 5-HT[2A] Receptor

Abstract

Studies indicate that 5-hydroxytryptamine (5-HT) released from activated platelets in coronary artery bypass grafting (CABG) induces 5-HT_2A receptor-mediated graft spasm. We previously reported that 5-HT-induced constriction of human endothelium-denuded saphenous vein (SV) was significantly augmented in patients with diabetes mellitus (DM) than in patients without DM (non-DM), without changes in the levels of the membrane-bound 5-HT_2A receptor of their smooth muscle cells. Although the internal thoracic artery (ITA) is the key graft conduit for CABG, the effect of DM on the ITA graft spasm is still unclear. Therefore, in this study, we investigated the effect of DM on 5-HT-induced vasoconstriction and the level of membrane-bound 5-HT_2A receptor in ITA grafts. 5-HT-induced constriction of the isolated human endothelial-denuded ITA was significantly higher in patients with DM than in patients without DM. In addition, the level of the 5-HT_2A receptor in the membrane fraction of human ITA smooth muscle cells was significantly higher in patients with DM than in those without DM. These results demonstrate that DM is a risk factor for CABG in both venous and arterial conduits, and that it differentially affects the level of the membrane-bound 5-HT_2A receptor in the venous and arterial smooth muscle cells.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 41 (5), 820-823, 2018-05-01

  The Pharmaceutical Society of Japan

Keywords

• 5-hydroxytryptamine
• coronary artery bypass grafting
• diabetes mellitus
• internal thoracic artery
• vasospasm

Details

• CRID: 1390282679608780032
• NII Article ID: 130006730769
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.b17-01000
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 028958840
• PubMed: 29434166
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028958840
• Web Site: https://www.jstage.jst.go.jp/article/bpb/41/5/41_b17-01000/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed