Variation in the Phenotype of Photosensitive Cells Produced from Human Fibroblast Cell Lines
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- Ishii Toshiyuki
- Department of Physiology, Nippon Medical School
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- Yin Chengzhu
- Department of Physiology, Nippon Medical School
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- Seko Yuko
- Visual Functions Section, Department of Rehabilitation for Sensory Functions, Research Institute, National Rehabilitation Center for Persons with Disabilities
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- Umezawa Akihiro
- Department of Reproductive Biology, Center for Regenerative Medicine, National Institute for Child Health and Development
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- Kaneda Makoto
- Department of Physiology, Nippon Medical School
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<p>Background: Photoreceptors differentiated from somatic cells are a useful tool for transplantation and drug screening. We previously showed that photosensitive cells are differentiated from human fibroblasts by direct reprogramming. In induced pluripotent stem (iPS) cells or embryonic stem (ES) cells, the properties of differentiated cells differ among the source of cell lines. However, whether or not the properties of the photosensitive cells produced by direct reprogramming are controlled by the origin of the cell line remains unknown. Methods: We compared the morphological and physiological properties of photosensitive cells induced by two fibroblast cell lines. Results: The differentiated cells had larger somas and more primary processes than the non-infected cells in both cell lines. The degree of morphological change was statistically different between the two cell lines. In addition, physiological responses to light differed between the two cell lines. An outward current (photoreceptor-like response) was observed in both cell lines, while an inward current (intrinsically photosensitive retinal ganglion cell-like response) was observed only in one cell line under light stimulation. Conclusions: These results suggest that photosensitive cells produced from different cell lines by direct reprogramming might express different phenotypes.</p>
収録刊行物
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- 日医大誌
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日医大誌 85 (2), 110-116, 2018-04-15
日本医科大学医学会