Interplay between chromatin modulators and histone acetylation regulates the formation of accessible chromatin in the upstream regulatory region of fission yeast <i>fbp1</i>

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Author(s)

Abstract

<p>Numerous noncoding RNA transcripts are detected in eukaryotic cells. Noncoding RNAs transcribed across gene promoters are involved in the regulation of mRNA transcription via chromatin modulation. This function of noncoding RNA transcription was first demonstrated for the fission yeast <i>fbp1</i> gene, where a cascade of noncoding RNA transcription events induces chromatin remodeling to facilitate transcription factor binding. We recently demonstrated that the noncoding RNAs from the <i>fbp1</i> upstream region facilitate binding of the transcription activator Atf1 and thereby promote histone acetylation. Histone acetylation by histone acetyl transferases (HATs) and ATP-dependent chromatin remodelers (ADCRs) are implicated in chromatin remodeling, but the interplay between HATs and ADCRs in this process has not been fully elucidated. Here, we examine the roles played by two distinct ADCRs, Snf22 and Hrp3, and by the HAT Gcn5 in the transcriptional activation of <i>fbp1</i>. Snf22 and Hrp3 redundantly promote disassembly of chromatin in the <i>fbp1</i> upstream region. Gcn5 critically contributes to nucleosome eviction in the absence of either Snf22 or Hrp3, presumably by recruiting Hrp3 in <i>snf22</i>∆ cells and Snf22 in <i>hrp3</i>∆ cells. Conversely, Gcn5-dependent histone H3 acetylation is impaired in <i>snf22</i>∆/<i>hrp3</i>∆ cells, suggesting that both redundant ADCRs induce recruitment of Gcn5 to the chromatin array in the <i>fbp1</i> upstream region. These results reveal a previously unappreciated interplay between ADCRs and histone acetylation in which histone acetylation facilitates recruitment of ADCRs, while ADCRs are required for histone acetylation.</p>

Journal

  • Genes & Genetic Systems

    Genes & Genetic Systems 92(6), 267-276, 2017

    The Genetics Society of Japan

Codes

  • NII Article ID (NAID)
    130006731266
  • NII NACSIS-CAT ID (NCID)
    AA11077421
  • Text Lang
    ENG
  • ISSN
    1341-7568
  • NDL Article ID
    028987796
  • NDL Call No.
    Z53-W539
  • Data Source
    NDL  J-STAGE 
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