piggyBac- and phiC31 integrase-mediated transgenesis in Drosophila prolongata

DOI Web Site Web Site PubMed 被引用文献2件 参考文献31件
  • Kudo Ayumi
    Department of Agricultural and Environmental Biology, The University of Tokyo
  • Awasaki Takeshi
    Department of Biology, Kyorin University School of Medicine
  • Ishikawa Yukio
    Department of Agricultural and Environmental Biology, The University of Tokyo
  • Matsuo Takashi
    Department of Agricultural and Environmental Biology, The University of Tokyo

書誌事項

タイトル別名
  • <i>piggyBac</i>- and phiC31 integrase-mediated transgenesis in <i>Drosophila prolongata</i>

この論文をさがす

抄録

<p>The development of transgenesis systems in non-model organisms provides a powerful tool for molecular analysis and contributes to the understanding of phenomena that are not observed in model organisms. Drosophila prolongata is a fruit fly that has unique morphology and behavior not found in other Drosophila species including D. melanogaster. In this study, we developed a phiC31 integrase-mediated transgenesis system for D. prolongata. First, using piggyBac-mediated transgenesis, 37 homozygous attP strains were established. These strains were further transformed with the nosP-Cas9 vector, which was originally designed for phiC31-mediated transgenesis in D. melanogaster. The transformation rate varied from 0% to 3.4%. Nine strains with a high transformation rate of above 2.0% were established, which will serve as host strains in future transformation experiments in D. prolongata. Our results demonstrate that genetic tools developed for D. melanogaster are applicable to D. prolongata with minimal modifications.</p>

収録刊行物

被引用文献 (2)*注記

もっと見る

参考文献 (31)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ