Extracellular Bio-imaging of Acetylcholine-stimulated PC12 Cells Using a Calcium and Potassium Multi-ion Image Sensor

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Abstract

<p>In biochemistry, Ca<sup>2+</sup> and K<sup>+</sup> play essential roles to control signal transduction. Much interest has been focused on ion-imaging, which facilitates understanding of their ion flux dynamics. In this paper, we report a calcium and potassium multi-ion image sensor and its application to living cells (PC12). The multi-ion sensor had two selective plasticized poly(vinyl chloride) membranes containing ionophores. Each region on the sensor responded to only the corresponding ion. The multi-ion sensor has many advantages including not only label-free and real-time measurement but also simultaneous detection of Ca<sup>2+</sup> and K<sup>+</sup>. Cultured PC12 cells treated with nerve growth factor were prepared, and a practical observation for the cells was conducted with the sensor. After the PC12 cells were stimulated by acetylcholine, only the extracellular Ca<sup>2+</sup> concentration increased while there was no increase in the extracellular K<sup>+</sup> concentration. Through the practical observation, we demonstrated that the sensor was helpful for analyzing the cell events with changing Ca<sup>2+</sup> and/or K<sup>+</sup> concentration.</p>

Journal

  • Analytical Sciences

    Analytical Sciences 34(5), 553-558, 2018

    The Japan Society for Analytical Chemistry

Codes

  • NII Article ID (NAID)
    130006733524
  • NII NACSIS-CAT ID (NCID)
    AA10500785
  • Text Lang
    ENG
  • ISSN
    0910-6340
  • NDL Article ID
    028975921
  • NDL Call No.
    Z54-F482
  • Data Source
    NDL  J-STAGE 
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