Extracellular Bio-imaging of Acetylcholine-stimulated PC12 Cells Using a Calcium and Potassium Multi-ion Image Sensor

• MATSUBA Sota

  Department of Electrical & Electronic Information Engineering, Toyohashi University of Technology

• KATO Ryo

  Cooperative Research Facility Center, Toyohashi University of Technology

• OKUMURA Koichi

  Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology

• SAWADA Kazuaki

  Department of Electrical & Electronic Information Engineering, Toyohashi University of Technology Electronics-Inspired Interdisciplinary Research Institute, Toyohashi University of Technology

• HATTORI Toshiaki

  Department of Electrical & Electronic Information Engineering, Toyohashi University of Technology

Abstract

In biochemistry, Ca²⁺ and K⁺ play essential roles to control signal transduction. Much interest has been focused on ion-imaging, which facilitates understanding of their ion flux dynamics. In this paper, we report a calcium and potassium multi-ion image sensor and its application to living cells (PC12). The multi-ion sensor had two selective plasticized poly(vinyl chloride) membranes containing ionophores. Each region on the sensor responded to only the corresponding ion. The multi-ion sensor has many advantages including not only label-free and real-time measurement but also simultaneous detection of Ca²⁺ and K⁺. Cultured PC12 cells treated with nerve growth factor were prepared, and a practical observation for the cells was conducted with the sensor. After the PC12 cells were stimulated by acetylcholine, only the extracellular Ca²⁺ concentration increased while there was no increase in the extracellular K⁺ concentration. Through the practical observation, we demonstrated that the sensor was helpful for analyzing the cell events with changing Ca²⁺ and/or K⁺ concentration.

Journal

• Analytical Sciences

  Analytical Sciences 34 (5), 553-558, 2018-05-10

  The Japan Society for Analytical Chemistry

Keywords

• Bio-imaging
• ion image sensor
• multi-ion image sensor
• polyvinyl chloride membrane
• PC12 cell

Details

• CRID: 1390282679237893504
• NII Article ID: 130006733524
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.17p552
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 028975921
• PubMed: 29743426
• Web Site: http://id.ndl.go.jp/bib/028975921
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028975921
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.17P552.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.17P552/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/34/5/34_553/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed