Cellular Biological Validations of Proteomics Data

DOI
  • Kobayashi Daiki
    Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University
  • Araki Norie
    Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University

Bibliographic Information

Other Title
  • プロテオミクスデータの細胞生物学的な検証法

Abstract

<p>Cellular biological validations of novel candidate molecules which are extracted from proteomics data are needed to uncover their functions. For examples, the molecules are inhibited or activated to validate their biological functions by using siRNA/shRNA, antibodies, chemical compounds, or expression plasmids, followed by the observations with microscope and analyses using the various assay systems. However, it takes much effort to biologically validate the candidate molecules because the approaches depend on their various functions. Therefore, the precise processes to extract the novel molecules of biological interest using the gene ontology or network analyses, as well as the strict sample preparations and the reliable proteome data, need to facilitate their validations. In this paper, we explained how the novel candidates of interest, especially related to the neurofibromatosis type I (NF1) pathogenesis, were validated biologically.</p>

Journal

  • Proteome Letters

    Proteome Letters 1 (1), 37-43, 2016

    Japanese Proteomics Society

Keywords

Details 詳細情報について

  • CRID
    1390001206229546752
  • NII Article ID
    130006743371
  • DOI
    10.14889/jpros.1.1_37
  • ISSN
    24322776
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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