Identification of QTLs for rice grain size using a novel set of chromosomal segment substitution lines derived from Yamadanishiki in the genetic background of Koshihikari

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Author(s)

Abstract

<p>Grain size is important for brewing-rice cultivars, but the genetic basis for this trait is still unclear. This paper aims to identify QTLs for grain size using novel chromosomal segment substitution lines (CSSLs) harboring chromosomal segments from Yamadanishiki, an excellent sake-brewing rice, in the genetic background of Koshihikari, a cooking cultivar. We developed a set of 49 CSSLs. Grain length (GL), grain width (GWh), grain thickness (GT), 100-grain weight (GWt) and days to heading (DTH) were evaluated, and a CSSL-QTL analysis was conducted. Eighteen QTLs for grain size and DTH were identified. Seven (<i>qGL11</i>, <i>qGWh5</i>, <i>qGWh10</i>, <i>qGWt6-2</i>, <i>qGWt10-2</i>, <i>qDTH3</i>, and <i>qDTH6</i>) that were detected in F<sub>2</sub> and recombinant inbred lines (RILs) from Koshihikari/Yamadanishiki were validated, suggesting that they are important for large grain size and heading date in Yamadanishiki. Additionally, QTL reanalysis for GWt showed that <i>qGWt10-2</i> was only detected in early-flowering RILs, while <i>qGWt5</i> (in the same region as <i>qGWh5</i>) was only detected in late-flowering RILs, suggesting that these QTLs show different responses to the environment. Our study revealed that grain size in the Yamadanishiki cultivar is determined by a complex genetic mechanism. These findings could be useful for the breeding of both cooking and brewing rice.</p>

Journal

  • Breeding Science

    Breeding Science 68(2), 210-218, 2018

    Japanese Society of Breeding

Codes

  • NII Article ID (NAID)
    130006743984
  • NII NACSIS-CAT ID (NCID)
    AA11317194
  • Text Lang
    ENG
  • ISSN
    1344-7610
  • NDL Article ID
    029017417
  • NDL Call No.
    Z54-J372
  • Data Source
    NDL  J-STAGE 
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