Single-Particle Tracking Reveals a Dynamic Role of Actin Filaments in Assisting Long-Range Axonal Transport in Neurons

• Yasuko Osakada

• Kai Zhang

Abstract

Here, we demonstrated that actin filaments mediate axonal transport in dorsal root ganglia (DRG) neurons using fluorescence single-particle tracking. We employed a compartmentalized microfluidic cell culturing chamber that allows depolymerization of actin filaments within an axonal segment. We observed that local actin depolymerization results in a two-fold increase in the average pausing duration, whereas the microtubule-dependent instantaneous transport speed is not perturbed. Collectively, our data reveal an important role of actin filaments in assisting microtubule-dependent long-range NGF axonal transport in DRG neurons.

Journal

• Bulletin of the Chemical Society of Japan

  Bulletin of the Chemical Society of Japan 90 (6), 714-719, 2017-06

  Tokyo : Chemical Society of Japan

Keywords

• General Chemistry

Details

• CRID: 1523951030487848320
• NII Article ID: 130006770652
• NII Book ID: AA00580132
• ISSN: 00092673
• ISSN: 13480634
• DOI: 10.1246/bcsj.20170090
• NDL BIB ID: 028285631
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028285631
• Web Site: https://academic.oup.com/bcsj/article-pdf/90/6/714/56282788/bcsj.20170090.pdf
• Text Lang: en
• NDL Source Classification:
  • ZP1(科学技術--化学・化学工業)
• Data Source:
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN