Solubility-based Separation and Purification of Long-Chain Chitin Oligosaccharides with an Organic–Water Mixed Solvent
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- KATANO Hajime
- Department of Bioscience, Fukui Prefectural University
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- NOBA Shota
- Department of Bioscience, Fukui Prefectural University
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- SATO Kimihiko
- Koyo Chemical Co., Ltd.
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- KIMOTO Hisashi
- Department of Bioscience, Fukui Prefectural University
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抄録
A simple and rapid method for separation and purification of chitin oligosaccharides, (GlcNAc)n, with n ≥ 5 is presented. A commercially available chitin oligosaccharides sample, consisting of (GlcNAc)n with n = 1 – 7, was used as the starting material. Ten milligrams of the material was mixed with 100 μL of the 1 mol/L HCl. All the (GlcNAc)n species were dissolved in the aqueous medium. The aqueous solution was mixed with 900 μL of EtOH; the mixture was centrifuged, and the supernatant was removed to obtain a precipitate. The precipitate was found to consist mainly of (GlcNAc)n with n ≥ 5, indicating the significant difference in solubility between the short-chain (GlcNAc)n species with n ≤ 3 and the longer ones. By the repetition of the operations, a high purity long-chain (GlcNAc)n sample with n ≥ 5 could be prepared successfully. Since the long-chain (GlcNAc)n species are known to have excellent elicitor activity, this sample would be useful in the study of plant pathology, as well as chitin and chitosan chemistry.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 33 (5), 639-642, 2017
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679235932416
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- NII論文ID
- 130006777393
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- NII書誌ID
- AA10500785
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 028182161
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- PubMed
- 28496071
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可