Expression and protein localization analyses of Arabidopsis GLABRA3 (GL3) in tomato (Solanum lycopersicum) root epidermis
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- Tominaga-Wada Rumi
- Graduate School of Biosphere Sciences, Hiroshima University
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- Ota Kyosuke
- School of Applied Biological Science, Hiroshima University
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- Hayashi Naoto
- School of Applied Biological Science, Hiroshima University
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- Yamada Koh
- School of Applied Biological Science, Hiroshima University
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- Sano Ryosuke
- Graduate School of Biological Sciences, Nara Institute of Science and Technology
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- Wada Takuji
- Graduate School of Biosphere Sciences, Hiroshima University
書誌事項
- タイトル別名
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- Expression and protein localization analyses of <i>Arabidopsis GLABRA3</i> (<i>GL3</i>) in tomato (<i>Solanum lycopersicum</i>) root epidermis
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The arrangement of root hair and non-hair cells in the root epidermis provides a useful model for understanding the cell fate determination system in plants. A network of related transcription factors, including GLABRA3 (GL3), influences the patterning of cell types in Arabidopsis. GL3 is expressed primarily in root hair cells and encodes a bHLH transcription factor, which inhibits root hair differentiation in Arabidopsis root epidermis. By transforming the GL3 promoter::GFP into tomato, we demonstrated that the Arabidopsis GL3 promoter can function in tomato root epidermis. GFP fluorescence was observed in almost all root epidermal cells in the GL3::GFP transgenic tomato plants, indicating that all root epidermal cells of tomato possess root hair cell identity similar to that of Arabidopsis root hair cells. This is consistent with the phenotype of the tomato root, in which all epidermal cells produce root hairs. Moreover, we observed the localization of a GL3:GFP fusion protein in GL3::GL3:GFP transgenic tomato; although GL3 is known to exclusively localize in non-hair cell nuclei in Arabidopsis root epidermis, GL3:GFP fluorescence was detected not in the nuclei but in the cytoplasm of transgenic tomato epidermal cells. These results suggest that the nuclear localization mechanism differs between tomato and Arabidopsis.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 34 (2), 115-117, 2017
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204328195072
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- NII論文ID
- 130006789289
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- NII書誌ID
- AA11250821
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 028340198
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可