Predominant but Previously-overlooked Prokaryotic Drivers of Reductive Nitrogen Transformation in Paddy Soils, Revealed by Metatranscriptomics

• Masuda Yoko

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Itoh Hideomi

  Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center

• Shiratori Yutaka

  Niigata Agricultural Research Institute

• Isobe Kazuo

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Otsuka Shigeto

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Senoo Keishi

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Abstract

Waterlogged paddy soils possess anoxic zones in which microbes actively induce reductive nitrogen transformation (RNT). In the present study, a shotgun RNA sequencing analysis (metatranscriptomics) of paddy soil samples revealed that most RNT gene transcripts in paddy soils were derived from Deltaproteobacteria, particularly the genera Anaeromyxobacter and Geobacter. Despite the frequent detection of the rRNA of these microbes in paddy soils, their RNT-associated genes have rarely been identified in previous PCR-based studies. This metatranscriptomic analysis provides novel insights into the diversity of RNT microbes present in paddy soils and the ecological function of Deltaproteobacteria predominating in these soils.

Journal

• Microbes and Environments

  Microbes and Environments 32 (2), 180-183, 2017

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• paddy soils
• metatranscriptomics
• denitrification
• dissimilatory nitrate reduction to ammonium
• nitrogen fixation

Details

• CRID: 1390001204344299904
• NII Article ID: 40021235186
• NII Article ID: 130006789873
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me16179
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 028315530
• PubMed: 28442658
• Web Site: http://id.ndl.go.jp/bib/028315530
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I028315530
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/32/2/32_ME16179/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed