Quantitative Analysis of Protein Association System Using High-Pressure Electrophoresis
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- ISHIGURO Ryo
- Department of Chemistry and Biomolecular Science, Gifu University
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- FUJISAWA Tetsuro
- Department of Chemistry and Biomolecular Science, Gifu University
Bibliographic Information
- Other Title
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- 高圧力電気泳動法を用いたタンパク質解離会合系の定量的解析
- コウアツリョク デンキ エイドウホウ オ モチイタ タンパクシツ カイリ カイゴウケイ ノ テイリョウテキ カイセキ
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Abstract
High-pressure electrophoresis was designed to investigate the association system of proteins quantitatively. In this methodology, pressure and temperature precisely perturbs the association equilibrium of supermolecular protein assembly, whose decomposed fractions can be in-situ analyzed by native polyacrylamide gel electrophoresis (PAGE). The bands of these fractions allow us not only to unravel the elementary process of assembly formation but also to quantify various volumetric properties including thermal expansion change of each process. In this article, we report its application to the problem of amyloid protofibril formation.<br>
Journal
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- The Review of High Pressure Science and Technology
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The Review of High Pressure Science and Technology 27 (1), 26-32, 2017
The Japan Society of High Pressure Science and Technology
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Keywords
Details 詳細情報について
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- CRID
- 1390001204381302528
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- NII Article ID
- 130006792996
- 40021190521
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- NII Book ID
- AN10452913
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- ISSN
- 13481940
- 0917639X
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- NDL BIB ID
- 028171987
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed