Effect of TNF-α on MMP-1 and MMP-3 production in the fibroblast-like synoviocytes from human TMJ

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  • YANO Teruo
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • OGURA Naomi
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • KAWASHIMA Mutsumi
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • TAKAHASHI Kosuke
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • HATTORI Toshio
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • WATANABE Suguru
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • ISHIGAMI Daisuke
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • SUZUKI Mayu
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • SHIMIZU Hajime
    Department of Oral and Maxillofacial Surgery, Shonai Amarume Hospital
  • ITO Ko
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • KONDOH Toshirou
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

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Other Title
  • ヒト顎関節滑膜細胞におけるMMP-1およびMMP-3産生に対するTNF-<i>α</i>の影響

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Abstract

<p>Matrix metalloproteinase (MMP) plays a central role in tissue destruction of inflammatory joint disease. This study investigated gene expression of MMP-1, -3 and tissue inhibitors of metalloproteinase (TIMP) and protein production of MMP-1 and -3 in fibroblast-like synoviocytes (FLS) stimulated with TNF-α.</p><p>Method: Human synovial tissue was obtained from patients with internal derangement who underwent arthroscopy of the temporomandibular joint. FLSs were prepared from the tissues using the outgrowth method. TNF-α was added to the cells in culture. Gene expression profiling was performed using microarray analysis. Gene expression was examined using real-time PCR. Protein production was measured using enzyme-linked immunosorbent assay. APMA was used for the activation of pro-MMP-1.</p><p>Results: In microarray analysis of synovial cells, MMP-1 and -3 were recognized in the upregulated genes by TNF-α treatment. The gene expression of MMP-1 and -3 was also increased in FLS treated with TNF-α using real-time PCR. On the other hand, the genes of TIMP-1, -2 and -3 in FLS were constitutively expressed. The protein productions of pro-MMP-1 and MMP-3 increased in FLS treated with TNF-α. Although the level of active-MMP-1 in the conditional medium was below the measurement limit of ELISA Kit, the level of active-MMP-1 was increased in the conditional medium of FLS stimulated with TNF-α by APMA treatment.</p><p>Conclusion: TNF-α increased MMP-1 and -3 gene expression and pro-MMP-1 protein production in FLS.</p>

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