Detection of Fungi from an Indoor Environment using Loop-mediated Isothermal Amplification (LAMP) Method

• NAKAYAMA TAKAKO

  Division of Clinical Laboratory Medicine, Graduate School of Medical Care and Technology

• YAMAZAKI TAKASHI

  Laboratory of Space and Environmental Medicine, Graduate School of Medicine General Medical Education Research Center

• YO AYAKA

  Laboratory of Space and Environmental Medicine, Graduate School of Medicine

• TONE KAZUYA

  Laboratory of Space and Environmental Medicine, Graduate School of Medicine

• MAHDI ALSHAHNI MOHAMED

  General Medical Education Research Center

• FUJISAKI RYUICHI

  Emergency Room (ER), Department of Emergency -Medicine, Faculty of Medicine, Teikyo University

• MAKIMURA KOICHI

  Division of Clinical Laboratory Medicine, Graduate School of Medical Care and Technology Laboratory of Space and Environmental Medicine, Graduate School of Medicine General Medical Education Research Center

Abstract

　Loop-mediated isothermal amplification (LAMP) is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans (Ascomycota), Cryptococcus neoformans (Basidiomycota), and Mucor racemosus (Mucorales). The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction. In this study, we evaluated the correlation between colony forming units (CFU) and LAMP detection rate using the LAMP method for environmental fungi. The LAMP method should be a useful means of detecting fungi in indoor environments, disaster areas, or even in confined manned spacecraft to prevent allergies or infections caused by fungi.

Journal

• Biocontrol Science

  Biocontrol Science 22 (2), 97-104, 2017

  The Society for Antibacterial and Antifungal Agents, Japan

Keywords

• Environmental fungi
• Detection of fungi
• Loop-mediated isothermal amplification (LAMP)
• Procedure for Ultra Rapid Extraction (PURE)

Details

• CRID: 1390282679440295936
• NII Article ID: 130006832197
• DOI: 10.4265/bio.22.97
• ISSN: 18840205
• ISSN: 13424815
• PubMed: 28659561
• Web Site: https://www.jstage.jst.go.jp/article/bio/22/2/22_97/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018257915
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed