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- 西崎 浩
- 岡山大学医学部第二内科学教室
書誌事項
- タイトル別名
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- Interleukin-2 receptor α(p55) mRNA expression in alveolar lymphocytes of patients with sarcoidosis
- Interleukin-2 receptor α(p55) mRNA expression in alveolar lymphocytes of patients with sarcoidosis
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T-lymphocytosis was detected in bronchoalveolar lavage fluid of patients with sarcoidosis. To clarify the mechanism of this phenomenon, interleukin-2 receptor (IL-2R) α gene expression of lymphocytes recovered from bronchoalveolar lavage fluid was investigated in 8 patients with sarcoidosis and 5 healthy individuals, using reverse transcription polymerase chain reaction (RT-PCR). Cytoplasmic RNA derived from alveolar lymphocytes was reverse transcribed by RAV-2 reverse transcriptase to cDNA. The cDNA produced by reverse transcription was subjected to PCR. The primers of IL-2R α were utilized and a template derived from IL-2R mRNA was amplified by PCR. Southern blot analysis using 32P labeled cDNA probe for IL-2R α was performed followed by PCR. The intensities of IL-2R mRNA expression in Southern blot analysis were closely correlated to the cell numbers determined in RT-PCR.<br>All patients with sarcoidosis had higher expression of IL-2R α mRNA transcript in alveolar lymphocytes compared with healthy individuals. Moreover, the expression increased after stimulation by Propionibacterium acnes in 5 of 6 patients with sarcoidosis.<br>These findings suggest that alveolar T-lymphocytes in the patients with sarcoidosis were activated and played a central role in the pathogenesis of sarcoidosis.
収録刊行物
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- 岡山医学会雑誌
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岡山医学会雑誌 106 (1-2), 61-70, 1994
岡山医学会
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詳細情報 詳細情報について
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- CRID
- 1390001204873793536
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- NII論文ID
- 120002310283
- 130006856413
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- NII書誌ID
- AN00032489
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- ISSN
- 18824528
- 00301558
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