<i><b>Gm7325</b></i><b> is MyoD-dependently expressed in activated muscle satellite </b><b>cells </b>

• TAKEI Daisuke

  Graduate School of Pharmaceutical Sciences, Kyoto University

• NISHI Miyuki

  Graduate School of Pharmaceutical Sciences, Kyoto University Center for the Promotion of Interdisciplinary Education and Research, Kyoto University

• FUKADA So-ichiro

  Graduate School of Pharmaceutical Sciences, Osaka University

• DOI Masao

  Graduate School of Pharmaceutical Sciences, Kyoto University

• OKAMURA Hitoshi

  Graduate School of Pharmaceutical Sciences, Kyoto University

• UEZUMI Akiyoshi

  Tokyo Metropolitan Institute of Gerontology

• ZHANG Lidan

  Graduate School of Pharmaceutical Sciences, Osaka University

• YOSHIDA Morikatsu

  National Cerebral and Cardiovascular Center Research Institute

• MIYAZATO Mikiya

  National Cerebral and Cardiovascular Center Research Institute

• ICHIMURA Atsuhiko

  Graduate School of Pharmaceutical Sciences, Kyoto University Center for the Promotion of Interdisciplinary Education and Research, Kyoto University

• TAKESHIMA Hiroshi

  Graduate School of Pharmaceutical Sciences, Kyoto University

Abstract

The Gm7325 gene, bioinformatically identified in the mouse genome, encodes a small protein but has not been characterized until recently. Our gene expression analysis revealed that Gm7325 transcription is remarkably upregulated in injured skeletal muscle tissues. Activated satellite cells and immature myotubes were densely decorated with positive signals for Gm7325 mRNA in in situ hybridization analysis, while no obvious signals were observed in quiescent satellite cells and mature myofibers. In the 5’-flanking regions of mouse Gm7325 and its human homologue, conserved E-box motifs for helix-loop-helix transcription factors are repeatedly arranged around the putative promoter regions. Reporter gene assays suggested that MyoD, a master transcription factor for myogenesis, binds to the conserved E-box motifs to activate Gm7325 expression. Therefore, Gm7325, as a novel MyoD-target gene, is specifically induced in activated satellite cells, and may have an important role in skeletal myogenesis.

Journal

• Biomedical Research

  Biomedical Research 38 (3), 215-219, 2017

  Biomedical Research Press

Keywords

• General Biochemistry, Genetics and Molecular Biology
• General Medicine

Details

• CRID: 1390282679878954752
• NII Article ID: 130006865614
• DOI: 10.2220/biomedres.38.215
• ISSN: 1880313X
• ISSN: 03886107
• PubMed: 28637957
• Web Site: https://www.jstage.jst.go.jp/article/biomedres/38/3/38_215/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2018114701
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed