Preparation of polymorphonuclear leucocyte-plasma membranes which show Fc receptor activity.

• TSUNAWAKI Shohko

  Faculty of Pharmaceutical Sciences, Teikyo University

• MIZUNO Den'ichi

  Faculty of Pharmaceutical Sciences, Teikyo University

• KASAHARA Michihiro

  Department of Biology, Faculty of Science, The University of Tokyo

抄録

A plasma membrane-rich fraction was prepared from guinea pig peritoneal polymorphonuclear leucocytes (PMNs) by a nitrogen cavitation method. Fc receptor activity was measured in the fraction. The activity showed a K_d of 5×10^-7 M IgG and a maximum binding of 33 pmol IgG/mg protein when measured with an immune complex made with bovine serum albumin and rabbit anti-(bovine serum albumin) immunoglobulin G. Properties of the Fc receptor in the plasma membrane fraction were similar to that in intact PMNs. It is proposed that the Fc receptor activity and 5'-nucleotidase activity are markers for plasma membranes of these cells.

収録刊行物

• The Journal of Biochemistry

  The Journal of Biochemistry 92 (6), 1891-1900, 1982

  社団法人 日本生化学会

詳細情報

• CRID: 1572261552885284608
• NII論文ID: 130006866317
• Web Site: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=7161263&dopt=AbstractPlus
• 本文言語コード: en
• データソース種別:
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