Proteolytic cleavage of an activated subcomponent of the first component of rabbit complement, C1s.

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Author(s)

    • UEDA Emiko
    • Department of Microbiology, Faculty of Pharmaceutical Sciences, Higashi Nippon Gakuen University
    • TAKEUCHI Tsugio
    • Department of Microbiology, Faculty of Pharmaceutical Sciences, Higashi Nippon Gakuen University
    • TANIUCHI Sachiko
    • Department of Microbiology, Faculty of Pharmaceutical Sciences, Higashi Nippon Gakuen University
    • KOYAMA Jiro
    • Faculty of Pharmaceutical Sciences, Hokkaido University

Abstract

When rabbit C_??_ purified by affinity chromatography on IgG-Sepharose 6 B was chromatographed on DEAE-cellulose in the presence of ethylenediaminetetraacetate, C_??_s was isolated as two forms, C_??_s (I) and C_??_s (II), having different molecular weights. On the other hand, incubation of the C_??_ with soybean trypsin inhibitor before the chromatography resulted in the isolation of C_??_s (I) alone, indicating that, during the purification, C_??_s (II) was derived from C_??_s (I) by proteolytic cleavage of C_??_s (I) by a contaminating protease, probably plasmin [EC 3. 4. 21. 7]. In fact, C_??_s (I) was completely converted to C_??_s (II) or a C_??_s (II)-like fragment by highly purified plasmin. Analysis of the polypeptide chain structures revealed that C_??_s (I), which consisted of H and L chains with molecular weights of 70, 000 and 36, 000, respectively, was converted to C_??_s (II) by cleavage of the H chain, since C_??_s (II) consisted of two chains each with a molecular weight of 37, 000. This conversion proceeded without any alteration in C_??_ esterase activity, but was accompanied by loss of the ability to form C_??_r-C_??_s complex.

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 87(6), 1757-1763, 1980

    The Japanese Biochemical Society

Codes

  • NII Article ID (NAID)
    130006874019
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • ISSN
    0021924X
  • NDL Article ID
    2216907
  • NDL Source Classification
    SC141(生化学(医))
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z53-B472
  • Data Source
    NDL  J-STAGE 
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