Studies on Pyruvate Kinase (PK) Deficiency
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1. Previous findings that three types of pyruvate kinase [EC 2. 7. 1. 40] (PK) are present in human tissues were confirmed and these PK's were partially characterized. Their distribution in human tissues was almost identical to that in rat tissues. Results of electrophoretic, immunological, and kinetic studies on erythrocyte PK suggested that it might be a hybrid of L and M<sub>2</sub> subunits.<br>2. On the basis of these fundamental results, the PK in erythrocytes and other tissues (<i>i.e</i>., liver, muscle, spleen, and adipose tissues) of human subjects with two typical types of PK deficiency was analyzed. Case M.I. (PK “Beppu”) showed quantitative abnormality of erythrocyte PK and liver L-PK, while M<sub>1</sub> (muscle) and M<sub>2</sub> (spleen and adipose tissue) were normal. In cases Y. K. and F. K. (PK “Tokyo”), qualitative abnormality of erythrocyte PK and liver L-PK was found, while M<sub>1</sub> (muscle) and M<sub>2</sub> (spleen and adipose tissue) were normal as in case M.I.
- The Journal of Biochemistry
The Journal of Biochemistry 74(6), 1165-1175, 1973
The Japanese Biochemical Society